L-肉碱对糖尿病大鼠生精细胞凋亡及附睾精子数量和活动率的影响

目的:探讨L-肉碱(LC)对糖尿病(DM)大鼠生精细胞凋亡及附睾精子数量和活动率的影响。方法:24只雄性SD大鼠随机均分为3组,一组作为对照组,剩余两组分别注射链脲佐菌素(STZ,65 mg/kg)建立DM模型。建模成功后,各组大鼠分别给予如下灌胃剂量:对照组:生理盐水;DM模型组:生理盐水;LC组:300 mg/kgLC溶液,连续灌胃6周。末次给药24 h后,麻醉处死所有大鼠,分别进行附睾精子计数并检测精子活动率,流式细胞术检测各组大鼠睾丸生精细胞凋亡情况。结果:用LC治疗后的大鼠附睾头、尾精子活动率(%)分别为53.7±1.8和60.3±1.6,显著高于DM模型大鼠(分别为32.2±2.0和40.5±1.4,P<0.05),但低于对照组大鼠精子活动率63.1±2.4和68.9±1.3。与对照组附睾尾精子相对计数(37.8±1.1)×106/100 mg]相比,DM组显著减少(25.5±1.1)×106/100 mg],且具有统计学差异(P<0.05);LC治疗后大鼠附睾尾精子相对计数(32.0±1.5)×106/100 mg]比DM组显著增加(P<0.05),但仍低于对照组。与对照组生精细胞凋亡率(3.7±1.3)%]相比,DM组生精细胞凋亡率(52.5±4.4)%]显著上升(P<0.05);经LC治疗后,LC组大鼠生精细胞凋亡率为(35.3±3.5)%,比DM组显著降低(P<0.05),但仍显著高于对照组。结论:LC(300 mg/kg)灌胃DM大鼠6周,可以减少DM大鼠生精细胞凋亡,增加附睾精子数量,提高精子活动率。

Effects of L-carnitine on the apoptosis of spermatogenic cells and epididymal sperm count and motility in rats with diabetes mellitus

Objective： To explore the effects of L-carnitine（LC） on the apoptosis of spermatogenic cells and on the count and motility of epididymal sperm in rats with diabetes mellitus（DM）.Methods： Twenty-four SD rats（200-230 g） were randomly divided into a control group,a DM model group and an LC group.After the establishment of DM models in the latter two groups by injection of streptozotocin（STZ） at 65 mg/kg,the controls and DM models were treated intragastrically with physiological saline,while the rats in the LC group with LC at 300 mg/kg,all for 6 consecutive weeks.Twenty-four hours after the last administration,all the rats were killed for the detection of the count and motility of epididymal sperm and the apoptosis of spermatogenic cells.Results： The motilities of caput and cauda epididymal sperm were（53.7 ± 1.8）% and（60.3 ± 1.6）% in the LC group,significantly higher than in the DM model group（32.2 ± 2.0]% and 40.5 ± 1.4]%,P0.05）,but remarkably lower than in the control（63.1 ± 2.4]% and 68.9 ± 1.3]%,P0.05）.The count of cauda epididymal sperm was（25.5 ± 1.1） × 106/100 mg in the DM models,and was increased to（32.0 ± 1.5） × 106 / 100 mg after LC treatment（P0.05）,but still markedly lower than in the controls（37.8 ± 1.1] × 106/100 mg）（P0.05）.The apoptosis rate of spermatogenic cells was（52.5 ± 4.4）% in the DM model group,and it was reduced to（35.3 ± 3.5）% after LC administration（P0.05）,but still significantly higher than in the control group（3.7 ± 1.3]%）（P0.05）.Conclusion： Intragastrical gavage of LC at 300 mg/kg for 6 weeks increased the epididymal sperm count,improved sperm motility,and reduced the apoptosis of spermatogenic cells in rats with DM.