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小牛血清及维生素对小鼠生精上皮细胞冻存的影响
引用本文:李莲军,李德雪,张学明,岳占碰,文兴豪,罗秉坤.小牛血清及维生素对小鼠生精上皮细胞冻存的影响[J].中华男科学杂志,2002,8(4):244-246.
作者姓名:李莲军  李德雪  张学明  岳占碰  文兴豪  罗秉坤
作者单位:中国人民解放军军需大学动物科技系基础兽医学教研室,吉林,长春,130062
基金项目:国家自然科学基金资助 (3 9770 5 5 4)
摘    要:目的 :探讨维生素C(VC)、维生素E(VE)及小牛血清 (NBS)对冻存小鼠生精上皮细胞的影响。 方法 :在DMEM培养液 10 %二甲基亚砜 (DMSO)的基础上 ,分别添加 0~ 2 0 %NBS ,以及在DMEM培养液 10 %DMSO 10 %NBS的基础上 ,分别添加 15 0 μg/mlVC及 5 0 μg/mlVE,冷冻保存 7d龄小鼠生精上皮细胞 ,37℃水浴复苏 ,锥虫蓝染色检查细胞复苏率。 结果 :冻存液中分别含 0、5 %、10 %及 2 0 %NBS时 ,细胞复苏率分别为 83.4 %、84 .7%、85 .7%及 83.6 % ,各复苏率之间差异无显著性 (P >0 .0 5 )。冻存液中分别含 15 0 μg/mlVC及 5 0 μg/mlVE时 ,细胞复苏率分别为 88.0 %及 82 .9% ,其复苏率与未加维生素的冻存液组 (85 .7% )相比差异无显著性 (P >0 .0 5 )。 结论 :在冻存液中添加不同浓度的NBS及添加抗氧化剂VC或VE,对小鼠生精上皮单细胞冻存没有明显的保护作用 ,不能显著提高细胞复苏率

关 键 词:生精上皮细胞  冷冻保存  维生素C  维生素E  小牛血清  小鼠
文章编号:1009-3591(2002)04-0244-03
修稿时间:2001年11月5日

Effects of Neoborn Bull Serum and Vitamins on Cryopreservation of Mouse Seminiferous Epithelial Cells
Lian Jun LI,De Xue LI,Xue Ming ZHANG,Zhan Peng YUE,Xing Hao WEN,Bing Kun LUO.Effects of Neoborn Bull Serum and Vitamins on Cryopreservation of Mouse Seminiferous Epithelial Cells[J].National Journal of Andrology,2002,8(4):244-246.
Authors:Lian Jun LI  De Xue LI  Xue Ming ZHANG  Zhan Peng YUE  Xing Hao WEN  Bing Kun LUO
Institution:Department of Preclinical Veterinary Medicine, Faculty of Animal Science and Technology, Quartermaster University of PLA, Changchun, Jilin 130062, China. llian63@sohu.com
Abstract:OBJECTIVES: To investigate the effects of newborn bull serum(NBS), vitamin C and vitamin E on cryopreservation of mouse seminiferous epithelial cells. METHODS: The seminiferous epithelial cells from 7-day-old mice were cryopreserved in different freezing solutions. The cell recoveries were examined by Trypan blue exclusive staining after thawing. The freezing solutions composed of DMEM, 10% dimethylsulphoxide(DMSO), and 0, 5%, 10%, or 20% NBS, respectively, or composed of DMEM, 10% DMSO, 10% NBS, and 150 micrograms/ml vitamin C or 50 micrograms/ml vitamin E, respectively. RESULTS: The cell recoveries in freezing solution containing 0, 5%, 10%, or 20% NBS were 83.4%, 84.7%, 85.7% and 83.6%, respectively. There were no significant differences between them. The cell recoveries in freezing solution containing vitamin C or vitamin E were 88.0% and 82.9%, respectively. There was no significant differences compared with that in freezing solution containing 10% DMSO and 10% NBS. CONCLUSIONS: NBS, vitamin C and vitamin E have no significant protecting effects on mouse seminiferous epithelial cells, and can not significantly improve the cell recoveries.
Keywords:Seminiferous epithelia  Cryopreservation  Vitamin C  Vitamin E  Neoborn bull serum  Mouse
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