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| 锌对硝普钠损害精子的保护性作用研究 | |
| 引用本文： | 杨麦贵,杨阳,张竹映,郝晓柯,郑善銮,钟立辉,樊爱玲,徐葳.锌对硝普钠损害精子的保护性作用研究[J].中华男科学杂志,2004,10(7):530-532,537. |
| 作者姓名： | 杨麦贵 杨阳 张竹映 郝晓柯 郑善銮 钟立辉 樊爱玲 徐葳 |
| 作者单位： | 1. 第四军医大学,西京医院检验科,陕西,西安,7100332 2. 第四军医大学,学员旅,陕西,西安,710033 3. 中国铁道建筑总公司西安医院小儿科,陕西,西安,710016 |
| 摘 要： | 目的 :探讨锌对外源性一氧化氮 (NO)供体硝普钠 (SNP)损伤精子的保护性作用。 方法 :利用相差显微镜及单细胞凝胶电泳分析锌对SNP影响精子运动能力、精子头尾连接及精子DNA链断裂的保护性。 结果 :SNP对精子运动能力有明显的抑制作用 ,精子彗星细胞百分率显著增加 ,精子头尾连接稳定百分率降低 ,而锌离子能提高精子的运动功能 ,防止精子DNA链的断裂和精子头尾连接的解离。 结论 :锌对NO损伤精子有一定的保护作用 ,其机制可能与精子核染色质部位的巯基有关。 |
| 关 键 词： | 锌 精子 一氧化氮 硝普钠 DNA |
| 文章编号： | 1009-3591(2004)07-0530-04 |
| 修稿时间： | 2003年8月11日 |
Study of Zinc in Protecting Sperm from Sodium Nitroprusside Damage |
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| Yang Maigui,Yang Yang,Zhang Zhuying,Hao Xiaoke,Zheng Shanluan,Zhong Lihui,Fan Ailing,Xu Wei.Study of Zinc in Protecting Sperm from Sodium Nitroprusside Damage[J].National Journal of Andrology,2004,10(7):530-532,537. | |
| Authors: | Yang Maigui Yang Yang Zhang Zhuying Hao Xiaoke Zheng Shanluan Zhong Lihui Fan Ailing Xu Wei |
| Institution: | Department of Clinical Laboratory, Xijing Hospital, Xián, Shaanxi 710033, China. |
| Abstract: | Objective: To study the function of zinc in preventing human sperm from being damaged by sodium nitroprusside(SNP),an external NO donor. Methods: Analyses were made of the function of zinc in protecting sperm from being influenced by SNP in such aspects as sperm motility,head-tail connection and the breakage of sperm DNA chain by using phase-contrast microscope and single cell gel electrophoresis(SCGE). Results: Sperm motility was obviously inhibited by SNP. The percentage of comet cells increased significantly but the stability of sperm head-tail connection decreased. Zinc could promote sperm motility,protect the DNA chain and prevent the sperm head-tail connection from breaking. Conclusion: Zinc can protect sperm from being damaged by NO. Its mechanism may be related to the mercaptol group of sperm chromatin. |
| Keywords: | DNA |
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