增龄大鼠阴茎组织中一氧化碳含量与细胞凋亡的相关性研究

目的:通过研究不同月龄大鼠阴茎组织内细胞凋亡及CO含量变化,进一步探讨勃起功能障碍发生机制。方法:雄性Wistar大鼠24只分为3组(每组8只),成年组(8月龄)、老年组(16月龄)和衰老组(24月龄)。取阴茎组织用苏木精-伊红(HE)染色,原位末端转移酶标记法(TUNEL)检测细胞凋亡,改良双波长法检测CO含量。结果:增龄大鼠阴茎组织中细胞凋亡显著增多(成年组:4.38±1.06,老年组:9.38±1.69,衰老组:18.50±1.60),CO含量减少成年组:(12.19±0.87)nmol/μg,老年组:(7.93±0.63)nmol/μg,衰老组:(5.68±0.62)nmol/μg],组间差异均有显著性(P<0.05)。CO含量与细胞凋亡的变化呈现良好的负相关性(r=-0.889,P<0.01)。结论:增龄大鼠阴茎组织中细胞凋亡与CO含量呈负相关,CO可能通过调控细胞凋亡机制从而参与了雄性性功能障碍的发生。

Correlation of carbon monoxide with cell apoptosis in the penile tissue of aging rats

Abstract] Objective： To explore the pathogenesis of male sexual dysfunction by studying cell apoptosis and carbonate monox- ide （CO） contents in the penile tissue of rats at different ages. Methods ： We equally divided 24 male Wistar rats into an adult group （8 months old）, an elderly group （16 months old） and an aged group （24 months old）, and detected cell apoptosis by HE staining and TUNEL and CO levels in the penile tissues of different groups by modified dual-wavelength spectrophotometry. Results： Cell apoptosis increased with aging： 4.38 ±1.06 in the aduh, 9.38 ±1.69 in the elderly and 18.50 ±1.60 in the aged group, while the CO content decreased with aging： 12.19 ±0.87 in the aduh, 7.93 ±0.63 in the elderly and 5.68 ±0.62 in the aged group, both with statistically significant differences among the 3 groups （ P 〈 0.05 ）. The change of cell apoptosis showed a good negative correlation with that of the CO content （r = - 0. 889, P 〈 0.01 ）. Conclusion ： Cell apoptosis and CO content change in the penile tissue of aging rats, and CO may participate in the development of ED by regulating cell apoptosis. Natl J Androl, 2013, 19 （ 11 ） ： 972 - 976