肺纤维化对大鼠阴茎勃起功能的影响

目的:研究肺纤维化对大鼠勃起功能的影响及其机制。方法:12周雄性SD大鼠40只,随机分为4组:正常对照4周组(A组)、6周组(B组)和肺纤维化大鼠4周组(C组)、6周组(D组)各10只,分别用生理盐水(A、B组)及博莱霉素(5 mg/kg)气管内注入,饲养4周(A、C组)、6周(B、D组)后,测定大鼠血清睾酮、动脉血气分析、阴茎海绵体内压/平均颈动脉压(ICP/MAP),取阴茎标本测定NOS活性及cGMP含量,实时荧光PCR检测eNOS、iNOS和nNOS的mRNA在阴茎海绵体的表达,W estern印迹检测阴茎海绵体eNOS蛋白的表达。结果:电刺激的3 V,5 V C组ICP/MAP×100(16.37±2.19,27.19±3.18)较A组(30.78±2.66,50.09±6.97)显著降低(P<0.05),D组ICP/MAP×100,3 V,5 V(10.17±1.31,17.40±1.74)较B组(31.45±3.07,51.23±7.23)显著降低(P<0.05),D组ICP/MAP×100值较C组显著降低(P<0.05)。C组PaO2(75.50±13.87)mmHg较A组(103.80±6.88)mmHg显著降低(P<0.05),D组PaO2(83.60±5.50)mmHg较B组(102.70±5.77)mHg显著降低(P<0.05)。C组血清睾酮水平(391.1±264.7)ng/d l较A组(175.9±53.0)ng/d l显著升高(P<0.05),D组血清睾酮水平(745.4±408.8)ng/d l较B组(177.8±52.3)ng/d l显著升高(P<0.05),同时D组血清睾酮水平较C组显著升高(P<0.05)。C组NOS活性及cGMP含量(1.50±0.14)U/mg prot,(35.69±3.64)pmol/mg]较A组(2.66±0.39)U/mg prot,(51.10±7.22)pmol/mg]显著降低(P<0.05),D组NOS活性及cGMP含量(1.40±0.20)U/mg prot,(34.55±4.30 pmol/mg)]较B组(2.75±0.36)U/mg prot,(52.15±6.86)pmol/mg]显著降低(P<0.05),C组与D组比较NOS活性及cGMP含量无显著性差异(P>0.05)。C组eNOS蛋白表达量(0.79±0.01)较A组(0.87±0.01)显著降低(P<0.01),D组eNOS蛋白表达量(0.71±0.02)较B组(0.88±0.01)显著降低(P<0.05),D组较C组eNOS蛋白表达量显著降低(P<0.05)。C组eNOS mRNA表达量(4.46±0.92)较A组(2.61±0.68)显著升高(P<0.05),D组eNOS mRNA(2.79±0.60)表达量与B组(2.69±0.65)无显著性差异(P>0.05),nNOS及iNOS的mRNA表达量在A、B组与C、D组间均无显著性差异(P>0.05)。结论:肺纤维化可通过抑制阴茎海绵体eNOS蛋白的表达、降低总NOS活性及cGMP含量等机制抑制阴茎勃起功能。

Pulmonary fibrosis induces erectile dysfunction in rats

Objective: To study the impact of pulmonary fibrosis on erectile function in rats and its mechanism.Methods: Forty 12-week-old healthy male SD rats were randomly divided into Groups A(4-week pulmonary fibrosis),B(6-week pulmonary fibrosis),C(4-week control,and D(6-week control).The models of pulmonary fibrosis were established by injection of bleomycin at 5 mg/kg in the trachea,while the controls were injected with normal saline only.At 4 and 6 weeks,all the rats were subjected to determination of the serum...