PFKFB3基因在前列腺癌中的表达及其对前列腺癌细胞糖酵解及生长的影响

目的探讨6-磷酸果糖激酶-2/果糖双磷酸酶-2同工酶3（PFKFB3）基因在前列腺癌中的表达及其对前列腺癌细胞糖酵解及生长的影响。 方法收集我院病理科前列腺增生和前列腺癌蜡块组织，应用免疫组化技术检测PFKFB3的表达水平。通过荧光定量PCR和Western blot实验检测正常前列腺上皮细胞（RWPE-1）和四种前列腺癌细胞系（PC3、LNCaP、DU145、C4-2）中PFKFB3的表达。应用RNA干扰技术敲低PFKFB3表达，采用细胞糖酵解试剂盒、CCK-8和克隆形成实验检测PFKFB3对前列腺癌细胞的糖酵解和增殖活性的影响。 结果与前列腺增生组织相比，前列腺癌组织中的PFKFB3表达量明显增高（59.7±0.25） vs （3.08±0.16），P<0.05]，且病理Gleason评分越高，PFKFB3表达量也越高。同样PFKFB3在不同前列腺癌细胞系中均明显高表达。抑制PFKFB3基因表达后，前列腺癌细胞的糖酵解和增殖能力显著降低。 结论PFKFB3基因在前列腺癌恶性进展中表达上调，促进肿瘤细胞的糖酵解和增殖，靶向PFKFB3可能为前列腺癌分子诊断和治疗提供潜在的应用价值。

Expression of PFKFB3 in prostate cancer and its effect on glycolysis and growth of prostate cancer cells

ObjectiveTo investigate the expression of 6-phosphofructo-2-kinase /fructose-2,6-biphosphatase 3 (PFKFB3) in prostate cancer and its effect on glycolysis and growth of prostate cancer cells. MethodsParaffin embedded tissues of benign prostatic hyperplasia (BPH) and prostate cancer in the Department of Pathology in our hospital were collected, and the expression level of PFKFB3 was detected by immunohistochemistry. PFKFB3 expression in benign prostatic epithelial cells (RWPE-1) and four prostate cancer cell lines (PC3, LNCaP, DU145, C4-2) was detected by fluorescence quantitative PCR and Western blotting. RNA interference was used to knock down the expression of PFKFB3, and the effects of PFKFB3 on the glycolysis and proliferation of prostate cancer cells were detected by cell glycolysis kit, CCK-8 and clonal formation assay. ResultsThe expression level of PFKFB3 in prostate cancer tissues is significantly higher than that in benign prostatic hyperplasia tissues (5.97±0.25) vs (3.08±0.16), P<0.05], and the expression level of PFKFB3 increases with the higher pathological Gleason score. PFKFB3 is also highly expressed in different prostate cancer cell lines. Inhibition of PFKFB3 significantly reduces glycolysis and proliferation of prostate cancer cells. ConclusionPFKFB3 gene is up-regulated in the malignant progression of prostate cancer and promotes glycolysis and proliferation of tumor cells. Targeting PFKFB3 may provide potential application value for molecular diagnosis and treatment of prostate cancer.