自制稀土永磁夹板对骨折愈合作用的观察

目的观察稀土永磁夹板对家兔骨折愈合质量及骨形态发生蛋白（BMP-2）、血管内皮细胞生长因子（VEGF）表达情况的影响,从分子生物学水平探讨磁场促进骨折愈合的作用机制。方法健康成年家兔50只,建立双前肢桡骨中段骨折模型。左侧用稀土永磁夹板固定,作为实验侧;右侧用内镶铅片小夹板固定,作为对照侧。分别于术后1、2、3、4、6周摄双前肢X线片后取材,进行HE及BMP-2、VEGF免疫组化染色并在光镜下观察。结果X线片图像定量分析结果比较,实验侧愈合明显快于对照侧。两侧差异有显著性（P〈0.05）。实验侧在1、2、3、4周VEGF的表达均比对照侧强,两侧差异有显著性（P〈0.05）。实验侧在2、3、4周BMP-2的表达均比对照侧强,两侧差异有显著性（P〈0.01）。结论静磁场在骨折愈合过程中可加强BMP-2、VEGF的表达。自制稀土永磁夹板对实验性骨折愈合有明显的促进作用。

Observation on effect of the self-made magnetic clipboard of sparse soil on fracture healing

Objective To study the effect of the self-made magnetic clipboard of sparse soil on fracture healing and expression of the bone morphogenetic proteins and vascular endothelial growth factor in rabbits to investigate the biomechanism of influence of magnetic field on fracture healing.Methods Fifty health adulthood domestic rabbits were used to establish two forelegs mid-radius fracture model.The left side were fixed with sparse soil magnetic clipboard for fixing,being experiment side;The right side were fixed with unmagnetic clipboard,being the control side.Respectively X-ray, HE and BMP-2,VEGF Immune histochemistry dying were conducted to evaluate the fracture healing 1 week,2 weeks,3 weeks,4 weeks,6 weeks after operation.Results The result from the X-ray quantitative analysis revealed that the fracture healing of experimental side was obviously quicker than the control side,which showed a significant difference(P<0.05). The expression of VEGF in experiment group was all higher than it in control group at 1,2,3 and 4 weeks, showing a significant difference(P<0.01) The expression of BMP-2 are all higher than it in control group at 2,3,4 weeks ,showing a significant difference(P<0.01).Conclusions The quiet magnetic field can obviously accelerate the expression of BMP-2, the VEGF in the fracture healing.Self-made magnetic clipboard of sparse soil can obviously accelerate the fracture healing.