高糖环境下舒洛地特对大鼠近端肾小管上皮细胞增殖及细胞间黏附分子1表达的影响

目的探讨舒洛地特对高糖培养的大鼠近端肾小管上皮细胞（NRK52E）增殖和细胞间黏附分子1（ICAM-1）表达的影响。方法将NRK52E细胞用5．6mmol／L葡萄糖（NG组）、25mmol／L葡萄糖（HG组）、25mmol／L葡萄糖联合不同浓度舒洛地特（终浓度分别为0．5LRU／ml、1．0LRU／ml、2LRU／ml）于96孔板中分别培养24h、48h、72h后，运用MTT法测定细胞增殖变化。24h后，运用RT-PCR和Western blotting方法检测ICAM-1mRNA和蛋白质的表达。结果HG组细胞增殖及ICAM-1的表达均增强，舒洛地特组能抑制这种趋势并且呈剂量依赖性。结论舒洛地特能通过减少ICAM-1的表达而起到保护肾脏的作用。

Effect of sulodexide on cell proliferation and expression of intercellular adhesion molecule-I in NRKS2E cells induced by high glucose

Objective To explore the effects of sulodexide on proliferation and the expressions of Intercellular adhesion molecule-1 （ICAM-1） in NRK52E cells induced by high glucose. Methods The NRK52E cells were cultured with 5.6 mmol/L glucose （NG group）, 25. 0 mmol/L glucose（HG group）, HG and different concentrations of soludexide（0. 5,1. 0, and 2. 0 LRU/ml）, respectively. After culture for about 24,48,72 h, the proliferation activity of NRK52E cells was observed by MTT. Twenty-four hour later the expression of ICAM-1 was detected by RT-PCR and Western blotting. Results The proliferation activity and the expression of ICAM-1 in NRK52E cells were increased in HG group, but decreased in sulodexide group in a dose-independence manner. Conclusions Sulodexide maybe protect the kidney by decreasing the expression of ICAM-1.