儿茶酚胺对人前脂肪细胞增殖与分化的作用

目的 研究去甲肾上腺素、肾上腺素和异丙肾上腺素这三种儿茶酚胺对人前脂肪细胞增殖与分化的作用。方法 培养人皮下脂肪的前脂肪细胞，在其增殖与分化的不同阶段分别加入肾上腺素、去甲肾上腺素和异丙肾上腺素这三种儿茶酚胺，观察其对人前脂肪细胞的增殖，分化过程中的磷酸甘油脱氢酶(GPDH)和细胞内脂肪积聚的作用，并探讨α受体和β受体阻断剂对这个过程的影响。结果:肾上腺素和异丙。肾上腺素对人前脂肪细胞的增殖有促进作用，对分化过程中的GPDH的升高有抑制作用；而对于分化过程中的脂肪积聚三者均有促进分解的作用；均通过前脂肪细胞的β受体完成。结论 人前脂肪细胞对儿茶酚胺的反应与成熟脂肪细胞相比有其特点，可望应用于脂肪分解药物的研究。

Effects of catecholamines on human preadipocyte proliferation and differentiation

OBJECTIVE: To study the effects of catecholamines on human preadipocyte proliferation and differentiation. METHODS: Catecholamines (epinephrine, isoprenaline and noradrenaline) were added to the culture media of human preadipocytes. The proliferation of cells, the expression of GPDH and lipid droplet accumulation in cytoplasm were observed and recorded. The functions of alpha and beta receptors were examined with adding alpha and beta receptor blockers. RESULTS: Epinephrine and isoprenaline stimulated human preadipocyte proliferation and inhibited GPDH up-regulation during differentiation. The three types of catecholamines inhibited lipid accumulation in cell differentiation. The beta-adrenoceptors played a key role during the process. CONCLUSION: Human preadipocytes responded to catecholamine characteristically. The result would be applicable in the study of drugs for obesity.