急性脊髓损伤动物模型的建立

目的：建立一种不同程度的脊髓损伤动物模型。方法：自行设计一种脊髓损伤动物模型，以一恒定的外力和打击时间，以观察35只大鼠在不同的打击深度下的运动功能和病理变化。结果：假手术组大鼠，苏醒后能站立行走，脊髓结构正常；打击深度为0.5mm时，动物苏醒后能站立行走，脊髓结构正常，打击度为0.8mm 时，动物出现不瘫，数天后能站立行走，脊髓结构破坏较轻；打击深度为1．0 mm时，动物出现全瘫，4周后不能站立行走，脊髓结构破坏，打击深度为1．5 mm时，动物出现全瘫，4周后不能站立行走，脊髓结构破坏严重。结论：以打击深度为参数建立不同程度的脊髓损伤动物模型，重复性好较

ANIMAL MODEL OF ACUTE SPINAL CORD INJURY IN RATS

Objective To investigate a animal model of spinal cord injury in different degrees of impact. Methods A new weight drop device was designed with the character of controlled degree of impact and time. After thirty five rats underwent different degrees of impact, their motor function and pathological changes were observed. Results In control group, the rats could walk after reviving, and the micro structure of spinal cord was normal. With 0.5mm depth of impact, the rats also could walk, and the micro structure of spinal cord did not change obviously. With 0.8mm depth of impact, the rats could walk after several days of injury and only slight damage could be found in spinal cord. When the impact depth increased to 1.0 or 1.5mm, the rats were paralyzed completely and could not walk after four weeks of injury. Severe injury was observed in spinal cord . Conclusion This animal model of spinal cord injury is based on different degrees of impact. It has stable and repetitive characters for the research on spinal cord injury.