母体唾液及龈沟液细胞因子水平与早产低体重新生儿关系的病例对照研究

目的 验证牙周炎是否为早产低体重新生儿(preterm low birth weight,PLBW)母亲体内低度炎症的来源之一,初步探讨PLBW与炎症因子之间的关系.方法 以83例PLBW的母亲(PLBW组)及44名健康新生儿(normal birth weight,NBW)的母亲(NBW组)为研究对象,留取受试对象的静息全唾液、龈沟液,应用酶联免疫吸附试验检测唾液和龈沟液中白细胞介素(IL)-1β和IL-6水平,比较两组间唾液和龈沟液中细胞因子水平的差异,并分析细胞因子与分娩孕周、新生儿体重之间的相关性.结果 唾液IL-1β水平在PLBW组(78.32±11.81)ng/L]显著高于NBW组(39.66±11.89)ng/L],差异有统计学意义(P<0.05);分娩孕周与唾液IL-6之间呈显著的负相关关系(r=-0.274,P<0.01);新生儿体重与龈沟液IL-1β(r=-0.231,P<0.01)、唾液IL-6(r=-0.424,P<0.01)之间呈显著的负相关关系.结论 唾液和龈沟液中炎性因子水平越高,分娩孕周越短、新生儿体重越轻,母体牙周炎程度与PLBW之间可能存在一定程度的相关关系.

Association between preterm low birth weight and maternal cytokine levels of saliva and gingival crevicular fluid

Objective To examine the hypothesis that periodontitis was one of the sources of inflammation in preterm low birth weight (PLBW) mothers and investigate the relationship between PLBW and inflammatory factors.Methods The samples of saliva and gingival crevicular fluid (GCF) were collected from 83 PLBW mothers(PLBW group) and 44 normal birth weight(NBW) mothers(NBW group).The levels of IL-1β and IL-6 were analyzed by enzyme-linked immunosorbent assay.The concentrations of cytokines in saliva and GCF were compared between the two groups.Results IL-1β level in saliva of PLBW group was higher than which of NBW group significantly(78.32±11.81) ng/L vs.(39.66±11.89) ng/L, P<0.05].There was no statistically significant difference on IL-6 level in saliva(17.45±3.13) ng/L vs.(16.28±0.66) ng/L], IL-1β level in GCF(93.58±13.22) ng/L vs.(81.38±9.28) ng/L]and IL-6 level in GCF(15.35±0.28) ng/L vs.(14.87 ±0.37) ng/L) between two groups, but those of PLBW group were higher than NBW group.Significant negative correlations existed between gestational age at delivery and probing depth.It was also found significant negative correlations between gestational age at delivery and IL-6(r =-0.274, P<0.01), between the weight of the new bom infants and IL-6 levels in saliva(r =-0.424, P<0.01), IL-1β level in GCF(r =-0.231, P<0.01).Conclusions There was a tendency presenting the higher inflammatory cytokine in GCF and saliva, the shorter gestation period and the lower birth weight The levels of cytokine in saliva and GCF were higher in PLBW mothers.The correlation may be exist between maternal periodontitis and PLBW.