软组织在炎症状态下对不同基台材料反应的组织学观察

目的:比较种植体周围软组织对不同基台材料的组织学反应。方法:构建钛和氧化锆愈合基台修复的犬动物模型,并构建种植体周围黏膜炎模型,采集临床牙周指标:菌斑指数、牙龈指数和探诊深度,并对种植体周围的软组织进行采样,对其中的炎性细胞进行免疫组化染色观察和分析。结果:钛和氧化锆愈合基台周围的牙周指标相似,愈合基台周围软组织中的炎性细胞主要位于基底层、靠近愈合基台的软组织和附近的小血管,两种愈合基台周围在炎性状态下的炎性细胞浸润相似。结论:种植体周围的软组织在炎症状态下对钛和氧化锆基台的组织学反应相似。

Histological Observation of Peri-implant Soft Tissues to Different Implant Abutment Materials under Inflammatory Condition: a Study in Vivo

Objective: To observe the histological response of peri-implant soft tissues to different implant abutments materials with or without peri-implant mucotitis. Methods: The third and fourth premolars and the first molar of three beagle dogs were extracted and eight implants were placed into each dog after healing. Two months later, the titanium and zirconium healing abutments were connected, plaque index, gingival index and probing depth were recorded as the initial time. Then ligaments were placed around the neck of healing abutments to induce peri-implant mucotitis. Plaque index, gingival index and probing depth were recorded again. The soft tissues were harvested and histological observation was performed to evaluate the inflammatory cells infiltration around titanium and zirconium healing abutments. Results: There were no differences on plaque index and probing depth of soft tissues around titanium and zirconium healing abutments with peri-implantitis, but gingival index was significantly higher with peri-implantitis. The HE staining showed that the inflammatory cells were located in the soft tissues around healing abutments with peri-implantitis. The immunohistochemistry revealed that inflammatory cells were expressed at the basement memberane zone, soft tissues around titanium and zirconium healing abutments, and endothelial cells of vessels in the vicinity. Conclusion: Histological responses of peri-implant soft tissues to titanium and zirconium healing abutments were similar under inflammatory condition.