种植体表面TiO2/HA梯度涂层的生物相容性研究

目的：通过观察大鼠成骨细胞（osteoblast,OB）在微弧氧化（micro-arc oxidation,MAO）和水热处理形成的TiO2/HA梯度涂层表面的粘附和增殖等情况,评价微弧氧化陶瓷膜表面的生物相容性及微弧氧化-水热处理工艺应用于钛植入材料表面处理的可行性。方法：将纯钛试件共60枚分为微弧氧化处理组（M组）、微弧氧化-水热处理组（M-H组）和纯钛对照组。采用扫描电镜（scanning electron microscopy,SEM）观察表面形貌并用X线衍射（X-ray diffraction,XRD）对其进行成分分析。然后对不同时间点OB细胞在各组材料表面的附着、生长、增殖情况以及碱性磷酸酶（alkaline phosphatase,ALP）活性进行检测,并对实验结果进行统计分析。结果：微弧氧化处理后试件表面呈现多孔状,主要为锐钛矿型TiO2,也包含金红石型TiO2。经过后续水热处理,从扫描电镜照片可以看到试件表面析出一层白色柱形结晶体,同时XRD谱线出现了羟基磷灰石的衍射峰。试件与OB细胞共培养后可见细胞在材料周围生长良好,培养5d后,改性两组的材料表面细胞增殖比纯钛组及空白对照组明显增多。培养7d后,M组和M-H组细胞ALP活性大幅提高,各组间比较均有统计学差异。结论：MAO及水热处理后的TiO2/HA梯度涂层表面能有效提高OB细胞的早期粘附、增殖及ALP活性。

Biocompatibility of the TiO2/HA Gradient Coatings with Osteoblast

Objective：To study the biocompatibility of osteoblast（OB）on pure titanium surface treated by micro-arc oxidation（MAO）and hydrothermal treatment.Methods：60 samples of pure titanium were prepared and separated into three groups：MAO treatment（M group）,MAO and hydrothermal treatment（M-H group）,Ti group without treatment.Attachment,proliferation and ALP activity of OB cultured on the titanium surfaces were assessed at different time points.All data were statistically analysed.Results：The ceramic film showed excellent biocompatibility with OB.Cell attachment on the M group and M-H group were higher than that on Ti group surface.OB proliferation and ALP activity were higher on M-H group surface than those on the other two surfaces（P0.05）.Conclusion：Titanium surface treated with MAO and hydrothermal treatment exhibit better biocompatibility to OB than that of pure titanium and may increase original cell attachment,proliferation and ALP activity.