大鼠同种异体骨髓间充质干细胞联合Bio-Oss骨粉-bFGF复合物促进拔牙窝愈合的micro-CT研究

目的:观察以Bio-Oss骨粉bFGF支架联合大鼠骨髓间充质干细胞(BMSCs)植入大鼠拔牙窝后在牙槽窝愈合过程中对牙槽骨微结构的影响.方法:取3周龄SD大鼠股骨全骨髓,自然贴壁分离BMSCs.选取36只6周龄SD大鼠,拔除上颌后牙,立即植入不同材料.按植入材料分为4组,即复合物组植入BMSCs联合Bio-Oss骨粉b...

Effect of rat allogeneic BMSCs-Bio-Oss-bFGF compound on tooth extraction healing:a micro-CT study

PRUPOSE:To investigate the effect of a compound of BMSCs-Bio-Oss-bFGF on microstructure of extraction sockets in rats.METHODS:Bone mesenchymal stem cells(BMSCs)were isolated from bone marrow of 3-week SD rats by adherent method.Maxillary posterior teeth of 366-week SD rats were extracted and materials were implanted into sockets according to grouping.The rats were divided into 4 groups:compound group with implanting BMSCs-Bio-Oss-bFGF compound,powder group with implanting Bio-Oss,BMSCs group with implanting BMSCs,and control group without implanting any materials.The sockets were scanned by micro-CT 4 weeks,12 weeks and 24 weeks after implantation.Two-way ANOVA was used to assess whether there was significant difference between groups with GraphPad Prism 6.0 software package.RESULTS:There was no significant difference among groups in bone mineral density(BMD),trabecular separation(Tb.Sp),trabecular thickness(Tb.Th),degree of anisotropy(DA),and trabecular number(Tb.N)4 weeks after implantation.By 12 weeks,BMD of compound group was significantly greater than those of BMSCs group,powder group and control group(P<0.05),and significantly greater than those of powder group and control group at 24 weeks(P<0.05).Tb.Th of compound group was significantly greater than that of BMSCs group at 12 and 24 weeks(P<0.05).DA had no significant difference among groups at 4,12,and 24 weeks(P>0.05).Tb.Sp of compound group was significantly smaller than those of powder group,BMSCs group and control group at 24 weeks(P<0.05).Tb.N of compound group was significantly greater than those of BMSCs group and control group(P<0.05).CONCLUSIONS:The compound of rat allogeneic BMSCs-Bio-Oss-bFGF improves socket healing.