| 下颌下腺脱细胞基质的制备及其胶原成分观察 |
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| 引用本文： | 邓春富,谭学新,周青,黄绍辉,徐中飞,王玉新.下颌下腺脱细胞基质的制备及其胶原成分观察[J].上海口腔医学,2004,13(6):528-530. |
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| 作者姓名： | 邓春富 谭学新 周青 黄绍辉 徐中飞 王玉新 |
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| 作者单位： | 中国医科大学第一临床学院,口腔颌面外科,辽宁,沈阳,110001 |
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| 基金项目： | 辽宁省社会发展基金(2003225007-8) |
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| 摘 要： | 目的:制备天然的下颌下腺脱细胞基质材料,观察脱细胞后下颌下腺的组织结构,并对其成分进行分析。方法:采用化学除垢剂法对SD大鼠的下颌下腺进行脱细胞处理,通过组织学及免疫组织化学对脱细胞基质材料进行观察及检测。结果:光镜下见下颌下腺细胞消失,基质成分呈筛网状。免疫组织化学证实,在SD大鼠下颌下腺脱细胞前后的组织中,Ⅰ、Ⅱ、Ⅲ型胶原均呈强阳性表达,而Ⅳ、Ⅴ型胶原呈弱阳性或阴性表达。结论:TritonX-100脱细胞处理的大鼠下颌下腺基质中,主要成分为Ⅰ、Ⅱ、Ⅲ型胶原蛋白。
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| 关 键 词： | 下颌下腺 脱细胞 免疫组化 胶原蛋白 |
| 文章编号： | 1006-7248(2004)06-0528-03 |
| 修稿时间： | 2004年5月21日 |
The Development and collagen ingredients analysis of submandibular gland acellular matrix |
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| DENG Chun-fu,TAN Xue-xin,ZHOU Qing,HUANG Shao-hui,Xu Zhong-fei,WANG Yu-xin..The Development and collagen ingredients analysis of submandibular gland acellular matrix[J].Shanghai Journal of Stomatology,2004,13(6):528-530. |
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| Authors: | DENG Chun-fu TAN Xue-xin ZHOU Qing HUANG Shao-hui Xu Zhong-fei WANG Yu-xin |
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| Institution: | Department of Oral and Maxillofacial Surgery, First Clinical Hospital of China Medical University, Shenyang 110001, Liaoning Province, China. |
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| Abstract: | PURPOSE: To develop submandibular gland acellular matrix and observe its structure and analyze its histological ingredients. METHODS: The fresh submandibular glands of SD mice were cell-extracted by chemical detergent and histological ingredients were analyzed by immunohistochemistry. RESULTS: Submandibular gland cells disappeared, matrix ingredients appeared network structure under microscopy. I, II and III type collagen proteins of submandibular gland before and after cell-extracted were positive under immunohistochemistry. CONCLUSIONS: The main ingredients of submandibular gland acellular matrix were I, II and III type collagen proteins. |
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| Keywords: | Submandibular gland Acellular Immunohistochemistry Collagen proteins |
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