RANKL、MMP-9和MMP-2在成釉细胞瘤中的表达及意义

目的：探讨RANKL、MMP-9和MMP-2与成釉细胞瘤骨吸收机制之间的关系。方法：应用免疫组化、荧光定量RT—PCR及Western印迹方法，分别在不同分子水平检测RANKL、MMP-2和MMP-9在成釉细胞瘤中的表达，所有实验数据均经SPSS11．0统计软件包进行分析处理，组间比较采用t检验。结果：在所有成釉细胞瘤标本中，3种方法均可检测到RANKL、MMP-2和MMP-9的表达。其中，RANKL各型成釉细胞瘤中均呈恒定的高表达，而荧光定量RT—PCR检测结果显示，MMP-2在基底细胞型成釉细胞瘤中的表达水平显著高于其他两型（P〈0．05）。结论：MMP-2、MMP-9及RANKL可能共同参与成釉细胞瘤引起的骨质吸收过程。与MMP-9相比，MMP-2可能与成釉细胞瘤的侵袭性更为相关。

The expression and significance of RANKL, MMP-9 and MMP-2 in ameloblastoma

PURPOSE:The purpose of this study was to investigate the relation between the expression of RANKL, MMP-2 and MMP-9 in ameloblastoma and bone resorption caused by ameloblastoma.METHODS:Immunohistochemistry, real time RT-PCR and Western blot were used to detect the expression of RANKL, MMP-2 and MMP-9 in diverse types of ameloblastoma.The data were analyzed using SPSS 11.0 Software package for Student's t test.RESULTS:In all cases, the expression of MMP-2, MMP-9 and RANKL were detected by the three methods mentioned above.The expression of RANKL was constantly high in diverse subtypes of ameloblastoma, and the expression of MMP-2 mRNA in basal cell ameloblastoma was significantly higher than that in the other two subtypes (P<0.05).CONCLUSIONS:MMP-2, MMP-9 and RANKL may collectively participate in the bone resorption process caused by ameloblastoma.Compared with MMP-9, MMP-2 may be more closely correlated with invasiveness of ameloblastoma.