新型骨组织工程支架材料生物相容性的体内研究

目的通过比较2种新型骨组织工程支架材料即聚消旋乳酸/聚乳酸－聚乙二醇－聚乳酸/磷酸三钙（A）、聚消旋乳酸/聚乳酸－聚乙二醇－聚乳酸（B）与对照组聚消旋乳酸（C）修复兔下颌骨缺损的效果，探讨新型可吸收性生物支架材料体内埋植的生物相容性。方法24只成年新西兰大白兔按取材时间随机分为4组。双侧下颌骨下缘形成15 mm×6 mm全层骨质缺损, 每一缺损作为一个实验单位。每组内按完全随机化设计植入实验材料和对照材料。术后2、4、8、12周取材行大体标本、X线、组织学观察及计算机图像分析。结果复合支架材料A、B与聚消旋乳酸相比，复合支架B生物相容性好，同期成骨量最大；复合支架A出现明显的异物肉芽肿反应。结论新型复合支架材料B生物相容性好，效果优于聚消旋乳酸，有可能成为一种较理想的支架材料。复合支架A不适宜作为骨组织工程生物支架材料。

Biocompatibility of new bone tissue engineering scaffolds in vivo

Objective To investigate the biocompatibility of new bone tissue engineering scaffolds, A：D, L-polylactic acid（PDLLA）/polylactic acid-polyethylene glycol-polylactic acid-polylactic acid（PLA-PEG-PLA）/Tricalcium phosphate and B： PDLLA/PLA -PEG -PLA in vivo， compared with PDLLA in repair of a rabbit mandibular body defect. Methods 24 New Zealand adult rabbits were divided into 4 groups randomly. 15 mm×6 mm defects were made surgically in the bilateral mandibular bodies and each hemi-mandible was assigned as an experimental unit. The defects were randomly repaired with scaffold materials in each group. Specimens obtained were evaluated with general observation, X-ray, histomorphology and computerized graphical analysis at 2, 4 , 8, 12 weeks after surgery. Results Compared with PDLLA, the new scaffold materials B showed biocompatibility. At the same time the quantity of new bone produced was much more than that in control group（P<0.05）. The new scaffold materials A showed the clear chronic granulomatous inflammation. Conclusion New scaffold material B had sound biocompatibility. It was much better than PDLLA. So it may be an ideal bone tissue engineering scaffold material. A is not adapted to be used as scaffold material.