血管内皮生长因子受体-2在人乳牙及年轻牙恒牙髓中的表达

目的：评估乳牙及年轻恒牙牙髓内的血管内皮生长因子受体-2（VEGFR-2）表达以及VEGFR-2阳性细胞的空间分布。方法：10颗健康的乳牙和10颗健康年轻恒牙采用抗VEGFR-2抗体进行免疫组化的方法并借助Image-Pro-Plux软件分析系统测量牙髓细胞中VEGFR-2的光密度值，进行统计学分析。结果：乳牙及年轻恒牙的牙髓血管内皮细胞对VEGFR-2的免疫染色均为阳性。在乳牙牙髓中VEGFR-2阳性细胞趋向于靠近成牙本质细胞层，年轻恒牙中VEGFR-2的免疫染色的空间分布更加一致。乳牙的牙髓血管内皮细胞胞浆中VEGFR-2免疫组化染色阳性，且表达高于年轻恒牙，差异有统计学意义（P〈0．05），结论：VEGFR-2在乳牙及年轻恒牙牙髓的血管内皮细胞均能表达，表明这些细胞能够对由血管内皮生长因子调节的形态变化及成活信号产生反应。

Vascular endothelial growth factor receptor-2 expression in the pulp of human primary and young permanent teeth

Objective：The purpose of this study was to evaluate the expression of vascular endothelial growth factor receptor-2 （VEGFR-2） in endothelial cells within the dental pulp of human primary and young permanent teeth and the spa- tial distribution of VEGFR-2-positive cells. Method： Nine sound primary teeth and 4 sound young permanent teeth were evaluated by immunohistochemistry with a human anti-VEGFR-2 antibody. Stained tissue sections were analyzed qualitatively under light microscopy. Result： Here we observed that endothelial cells of both primary and permanent teeth showed positive immunostaining for VEGFR-2. Notably, VEGFR-2-positive ceils in the primary teeth tended to be found close to the sub- odontoblastic layer, whereas the spatial distribution of VEGFR-2 immunostaining in the permanent teeth was more uniform. Conclusion： VEGFR-2 was expressed in the microvascular endothelial cells of both primary and young permanent teeth, which suggests that these cells are capable of responding to the morphogenetic and survival signals mediated by VEGF.