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Post-processing of DLP-printed denture base polymer: Impact of a protective coating on the surface characteristics,flexural properties,cytotoxicity, and microbial adhesion
Institution:1. Center of Oral Implantology, Stomatological Hospital, Southern Medical University, South Jiangnan Road No. 366, Guangzhou 510280, China;2. ADMiRE research center - Additive Manufacturing, intelligent Robotics, Sensors and Engineering, School of Engineering and IT, Carinthia University of Applied Sciences, Europastraße 4, 9524 Villach, Austria;3. Department of Prosthodontics, Geriatric Dentistry and Craniomandibular Disorders, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Aßmannshauser Str. 4–6, Berlin 14197, Germany;4. Department of Dental Surgery, Sechenov First Moscow State Medical University, Bolshaya Pirogovskaya Street, 19с1, Moscow 119146, Russia;5. Department of Prosthodontics at the Centre of Dentistry, Oral Medicine and Maxillofacial Surgery with Dental School, Tübingen University Hospital, Osianderstr. 2–8, Tübingen 72076, Germany
Abstract:ObjectivesTo investigate the effect of a protective coating on the surface characteristics, flexural properties, cytotoxicity, and microbial adhesion of vat-photopolymerization additive-manufacturing denture base polymers.MethodsThe specimens were additively manufactured using digital light processing (DLP). Specimen surfaces were coated with the same printed resin, and mechanical polishing was used for comparison. Surface topography, arithmetical mean height (Sa), and water contact angle values were measured. Furthermore, flexural strength (FS)/modulus and fractography were evaluated. Also, cytotoxicity was evaluated by an extract test. Finally, an adhesion test was used to investigate the adhesion of mixed oral bacteria to the specimens.ResultsThe Sa values in the polished (0.26 ± 0.08 µm) and coated (0.38 ± 0.14 µm) groups were significantly lower than in the untreated (2.21 ± 0.42 µm) and control (2.01 ± 0.37 µm) groups. The coating treatment resulted in a higher FS compared to the untreated surface (p = 0.0002). After the coating treatment, no significant differences were found in relative cell viability between the groups (p > 0.05). The quantitative results showed significantly higher bacterial adhesion in the untreated group than in the polished (p = 0.0047) and coated (p < 0.0001) groups.SignificanceThe surface characteristics and flexural properties were optimized by the protective coating. Also, the protective coating did not adversely affect cytocompatibility. Moreover, the coating treatment could effectively decrease oral bacteria adhering to the surfaces. Therefore, the protective coating treatment can be a less time-consuming alternative to mechanical polishing as a post-processing procedure for the digital denture.
Keywords:Vat photopolymerization  Additive manufacturing  Digital denture  Surface characteristics  Flexural strength  Cytotoxicity  Microbial adhesion
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