不同浓度氟化物对体外培养成釉细胞活性的影响

目的建立大鼠成釉细胞原代培养技术,观察不同浓度氟化钠对成釉细胞活性的影响,为研究氟斑牙的形成提供依据。方法取10¹5 d的Wistar大鼠磨牙牙胚组织进行原代培养,通过酶消化法,分离培养成釉细胞。加入不同浓度(0、0.4、0.8、1.6、3.2、6.4 mmol/L)的氟化钠作用于成釉细胞,分别培养24、48、72 h后,采用CCK-8法检测各组细胞的活性情况。结果①当氟化物浓度为0.4、0.8 mmol/L时,对成釉细胞的增殖有促进作用,且随着时间的增加而增强。②当氟化物浓度为1.6、3.2、6.4 mmol/L时,对成釉细胞的增殖有抑制作用,随着氟化钠浓度的增加,对细胞的抑制作用也逐渐增强,并且抑制作用随着时间的延长愈发明显。结论不同浓度的氟化物对体外培养成釉细胞的活性具有促进和抑制双向作用。

Influence of fluoride concentration on ameloblast activity in vitro culture

Objective To develop the primary culture technique of rat ameloblasts and observe the influence of different concentra- tions of sodium fluoride on ameloblast activity in vitro culture to furnish basis for the study of dental fluorosis formation. Methods Take 10 - 15 d old Wistar rat molar tooth germ tissue for primary culture. The enzymes digesting method was used to isolate and culture the ameloblasts. Then ameloblasts were exposed to different concentrations of NaF（0,0.4,0.8,1.6,3.2,6.4 mmol/L） for 24,48 and 72 h. CCK-8 was used to detect cell activity. Results The proliferation of ameloblasts was promoted by NaF at 0.4 and O. 8 mmol/L, and the effect ＇sas strengthened as t~me increased whereas it was inhibited b3~ 1.6,3.2 and 6.4 mmoVL NaF. With the increase of NaF concentration and the time, the inhibitory action was also strengthened and became more obvious. Conclusions Fluoride concentration has a biphasic effect on ameloblast activity in vitro culture：at low doses,it promotes cell proliferation while at high doses it has negative effects.