两种正畸力作用下大鼠牙周组织中胶原相关蛋白的表达

目的观察不同正畸力作用下大鼠牙移动过程中Ⅰ型胶原和基质金属蛋白酶-1（matrix metalloprotein-ase-1,MMP-1）、金属蛋白酶组织抑制因子-1（tissue inhibitor of metalloproteinase-1,TIMP-1）在上颌第一磨牙牙周组织中的表达变化。方法建立大鼠正畸牙移动模型,分为加力50 g组和加力150 g组,随机选择加力侧,对侧不加力作为对照组,分别于术后3、6、12 h和1、3、7、14 d处死,用免疫组织化学方法观察各组中Ⅰ型胶原和MMP-1、TIMP-1表达的变化。结果Ⅰ型胶原和MMP-1、TIMP-1在加力组的阳性表达明显强于对照组,且表达有时间依赖性。多元回归分析发现MMP-1、MMP-1与TIMP-1比值（MMP-1/TIMP-1）有与Ⅰ型胶原表达量呈负相关的趋势,TIMP-1有与牵张区Ⅰ型胶原表达量呈正相关的趋势。结论 MMP-1和TIMP-1参与了正畸牙周组织改建过程中Ⅰ型胶原代谢的调节,其表达的平衡性对维护牙周组织的生理健康、正常改建起着非常重要的作用。

Effect of Different Orthodontic Force on the Expression of Collagen Related Proteins in Periodontium of Rat

Objective To explore the rebuilding mechanism during the orthodontic tooth movement by investigating the variation of the expression of collagenⅠ,MMP-1 and TIMP-1 in rat periodontal tissues.Methods Orthodontic appliance was placed between the left maxillary first molar and maxillary central incisors of rats.Groups were made according to orthodontic force and tooth movement period of 3 h,6 h,12 h,1 d,3 d,7 d and 14 d.CollagenⅠ and MMP-1、TIMP-1 expression were examined with immunohistochemical technique and image analysis system.Results Under the application of mechanical force,the collagenⅠ and MMP-1,TIMP-1 expression in the periodontal tissues were more stringer stained than those of the controls,and manifested time-dependence.Multiple regression analysis results displayed MMP-1 and MMP-1/TIMP-1 showed negative correlative direction with collagenⅠ,and TIMP-1 showed positive correlative direction with collagenⅠ in the tension zones.Conclusion The results suggested that MMP-1 and TIMP-1 regulated the expression of collagenⅠ in the periodontal remodeling during tooth movement.The level of MMP-1/TIMP-1 played an essential role in maintaining the periodontal health and normal rebuilding.