Assessment of ultra-structure,viability and function of lipopolysaccharides-stimulated human dermal fibroblasts treated with chrysin and exosomes (in vitro study) |
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| Institution: | 1. Associate Professor of Oral Biology, Faculty of Dentistry, Ain Shams University, Cairo, Egypt;2. Professor of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt;3. Professor of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Badr University in Cairo, Badr City, Egypt;4. Associate Professor of Oral Biology, Faculty of Oral and Dental Medicine, Future University in Egypt, Cairo, Egypt |
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| Abstract: | BackgroundLipopolysaccharides (LPS) stimulate production of inflammatory cytokines. Chrysin is flavonoid beneficial for treatment of inflammatory conditions. Bone marrow mesenchymal stem cell (BM-MSC) exosomes have regenerative ability in different tissues.ObjectiveTo assess potential role of chrysin and BM-MSC exosomes on ultra-structure, viability and function of human dermal fibroblasts-adult (HDFa) stimulated by LPS.MethodsHDFa cells were divided into: Group I: Cells received no treatment. Group II: Cells were stimulated with LPS. Group III: LPS stimulated cells were treated with chrysin. Group IV: LPS stimulated cells were treated with exosomes.ResultsAfter 48 h, ultrastructural examination of HDFa cells in Group I revealed intact plasma membrane and numerous cytoplasmic organelles. Group II displayed destructed plasma membrane and apoptotic bodies. Group III showed intact plasma membrane with loss of its integrity at some areas. Group IV demonstrated intact plasma membrane that showed fusion with exosomes at some areas. Statistical analysis of MTT represented highest mean value of cell viability% in Group IV followed by Groups III, I and II respectively. Statistical analysis of enzyme-linked immunosorbent assay (ELISA) showed the highest mean value of interleukin-1β (IL-1β) was in Group II followed by Groups III, IV and I, while highest mean values of interleukin-10 (IL-10), nuclear factor-erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) proteins were in Group I, followed by Groups IV, III and II respectively.ConclusionsLPS have harmful consequences on ultra-structure, viability and function of HDFa cells. BM-MSC exosomes have better regenerative action on inflamed fibroblasts in comparison to chrysin. |
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| Keywords: | BM-MSC exosomes Chrysin Fibroblast MTT ELISA LPS"} {"#name":"keyword" "$":{"id":"k0035"} "$$":[{"#name":"text" "_":"lipopolysaccharides BM-MSCs"} {"#name":"keyword" "$":{"id":"k0045"} "$$":[{"#name":"text" "_":"bone marrow mesenchymal stem cells HDFa"} {"#name":"keyword" "$":{"id":"k0055"} "$$":[{"#name":"text" "$$":[{"#name":"__text__" "_":"Human"} {"#name":"hsp" "$":{"sp":"0 25"}} {"#name":"__text__" "_":"dermal"} {"#name":"hsp" "$":{"sp":"0 25"}} {"#name":"__text__" "_":"fibroblasts-adult ELISA"} {"#name":"keyword" "$":{"id":"k0065"} "$$":[{"#name":"text" "_":"enzyme-linked immunosorbent assay IL-1β"} {"#name":"keyword" "$":{"id":"k0075"} "$$":[{"#name":"text" "_":"Interleukin-1β IL-10"} {"#name":"keyword" "$":{"id":"k0085"} "$$":[{"#name":"text" "_":"Interleukin-10 Nrf2"} {"#name":"keyword" "$":{"id":"k0095"} "$$":[{"#name":"text" "_":"Nuclear factor-erythroid 2-related factor 2 HO-1"} {"#name":"keyword" "$":{"id":"k0105"} "$$":[{"#name":"text" "_":"Heme oxygenase-1 SD"} {"#name":"keyword" "$":{"id":"k0115"} "$$":[{"#name":"text" "_":"standard deviation p-value"} {"#name":"keyword" "$":{"id":"k0125"} "$$":[{"#name":"text" "_":"probability value |
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