胰岛素和小檗碱对Ⅱ型糖尿病大鼠钛种植体骨整合的影响

目的:研究胰岛素和小檗碱对Ⅱ型糖尿病大鼠种植体骨整合的影响.方法:53只SD大鼠,随机抽取10只,分入H组(健康组).剩余大鼠利用高糖饮食+小剂量链脲佐菌素(STZ)诱导,获得Ⅱ型糖尿病模型大鼠共40只,随机等分为B组(小檗碱组)、I组(胰岛素组)、IB组(胰岛素+小檗碱组)和N组(未治疗组).将100枚纯钛种植体植入大鼠双侧股骨干骺端.第7、32、38、66和101天测量大鼠的体重(BW)和空腹血糖值(GLU).术后10周,取大鼠股骨行Micro-CT扫描、VG染色及种植体拔出实验.观察硬组织切片;测量骨体积分数(BV/TV)、骨小梁厚度(Tb.Th)、骨小梁数目(Tb.N)、骨小梁间隙(Tb.Sp)、骨小梁容积率(TBV)、骨接触率(BIC)及最大拔出力(MPF).结果:BW和GLU:IB组和H组间无显著的统计学差异(P＞0.05);但IB组显著优于I组和B组;I组和B组优于N组(P＜0.05).骨质(BV/TV、Tb.Th、Tb.N、Tb.Sp)和骨整合(TBV、BIC、MPF):IB组和H组间无显著的统计学差异(P＞0.05);但IB组优于I组和B组;I组和B组优于N组(P＜0.05).结论:胰岛素和小檗碱联用可有效降低Ⅱ型糖尿病大鼠血糖,并促进种植体的骨整合.

The effects of insulin and berberine on the osseointegration of titanium implant in type H diabetic rats

Objective:To study the effects of insulin and berberine on osseointegration of titanium implant in type Ⅱ diabetic rats.Methods:10 out of 53 SD rats were randomly selected and selected into H group(healthy group)(n =10).The remaining rats were treated with high glucose diet plus low dose of streptozotocin(STZ) and 40 rats were induced into the models of type Ⅱ diabeties.The 40 dibetic rats were randomly divided into group B(berberine),group Ⅰ(insulin),group IB(insulin + berberine) and group N(without treatment).2 pure titanium implants were implanted into the bilateral femoral metaphysis of each rat.Mter 7,32,38,66 and 101 d,the body weight(BW) and fasting blood glucose(GLU) were measured.After 10 week,the rats were sacrifice,femoral metaphysis samples were subjected to Micro-CT scan,VG staining and implant pull-out test.Hard tissue slices were observed;bone volume fraction(BV/TV),Liang Houdu (Tb.Th),trabecular bone trabecular number(Tb.N),Liang Jianxi (Tb.Sp),trabecular bone trabecular bone volume (TBV),bone contact ratio(BIC) and the maximum pull-out strength (MPF) were measured.Results:There was no significant difference of BW and GLU between IB group and H group(P ＞ 0.05),but the BW and GLU of IB group was better than those of Ⅰ group and B group,Ⅰ group and B group were better than those of group N (P ＜ 0.05).BV/TV,Tb.Th,Tb.N,Tb.Sp,TBV,BIC and MPF of IB group were not statistically different from those of H group(P ＞0.05);but the indexes of IB group were higher than those of Ⅰ group and B group,those of Ⅰ group and B group were higher than those of N group (P ＜ 0.05).Conclusion:The combination of insulin and berberine can effectively reduce blood glucose and can promote osseointegration of titanium implants in type Ⅱ diabetic rats.