| GAL/GALR2促进涎腺腺样囊性癌细胞神经侵袭的研究 |
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| 引用本文： | 王珺,李欢,杨子桧,完颜超杰,张峰瑞,杨新杰,魏建华,雷德林.GAL/GALR2促进涎腺腺样囊性癌细胞神经侵袭的研究[J].实用口腔医学杂志,2021(2):149-153. |
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| 作者姓名： | 王珺 李欢 杨子桧 完颜超杰 张峰瑞 杨新杰 魏建华 雷德林 |
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| 作者单位： | 军事口腔医学国家重点实验室 |
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| 基金项目： | 国家自然科学基金(编号:8197101029,82002867)。 |
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| 摘 要： | 目的:探究甘丙肽(Galanin,GAL)和甘丙肽2型受体(GALR2)在涎腺腺样囊性癌(SACC)神经侵袭中的作用。方法:将SACC细胞与小鼠背根神经节(DRG)共培养。qRT-PCR、Western Blot检测SACC细胞GALR2的表达;CCK-8、流式细胞术、划痕、Transwell检测GAL对SACC细胞增殖、凋亡及迁移侵袭的影响;神经侵袭实验检测GALR2阻滞(M871)对SACC细胞神经侵袭的影响。结果:共培养SACC细胞GALR2表达显著升高(P<0.05);GAL促进了SACC细胞增殖、迁移侵袭,抑制凋亡(P<0.05);M871可抑制SACC细胞神经侵袭。结论:神经来源GAL可激活SACC细胞的GALR2,促进其神经侵袭。
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| 关 键 词： | 涎腺腺样囊性癌 嗜神经侵袭 甘丙肽 甘丙肽2型受体 |
GAL/GALR2 promotes the perineural invasion of salivary adenoid cystic carcinoma cells |
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| WANG Jun,LI Huan,YANG Zihui,WANYAN Chaojie,ZHANG Fengrui,YANG Xinjie,WEI Jianhua,LEI Delin.GAL/GALR2 promotes the perineural invasion of salivary adenoid cystic carcinoma cells[J].Journal of Practical Stomatology,2021(2):149-153. |
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| Authors: | WANG Jun LI Huan YANG Zihui WANYAN Chaojie ZHANG Fengrui YANG Xinjie WEI Jianhua LEI Delin |
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| Institution: | (State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi Clinical Research Center for Oral Diseases,Department of Oral and Maxillofacial Surgery,School of Stomatology,the Fourth Military Medical University,Xi'an 710032 China) |
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| Abstract: | Objective:To explore the role of galanin(GAL)and galanin receptor 2(GALR2)in perineural invasion(PNI)of salivary adenoid cystic carcinoma(SACC)cells.Methods:SACC cells were co-cultured with dorsal root ganglion(DRG).qRT-PCR and Western Blot were used to detect GALR2 expression in SACC cells.The effects of GAL on the proliferation apoptosis,migration and invasion of SACC cells were detected by CCK-8 assay,flow cytometry,wound healing scratch,and Transwell assays.The effect of GALR2 blocking M871 on PNI of SACC cells was detected by nerve invasion assay.Results:The expression of GALR2 in co-cultured SACC cells was increased(P<0.05).GAL promoted the proliferation,migration and invasion but inhibited the apoptosis of SACC cells(P<0.05).M871 inhibited the PNI of SACC cells.Conclusion:Neurogenic GAL may activate the expression of GALR2 in SACC cells and promote the PNI of the cells. |
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| Keywords: | Salivary adenoid cystic carcinoma Perineural invasion Galanin GALR2 |
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