牛蛙血细胞核酸和脊髓运动神经细胞的显色观察

目的建立牛蛙血细胞核酸及脊髓运动神经细胞显色方法,更好地服务教学和临床。方法制备临时装片,用福尔根反应和甲基绿-派洛宁对细胞内的核酸进行显色,用甲苯胺蓝染液对脊髓运动神经细胞进行染色。结果福尔根反应后,细胞核呈紫红色,细胞质呈绿色。该反应的最适条件为室温水解2 min,60℃水解8 min,再室温水解2 min,复染40 s。甲基绿-派洛宁染色后,细胞核呈蓝色,细胞质呈粉红色。甲苯胺蓝染色后,脊髓运动神经细胞被染成深蓝色。结论福尔根反应中亮绿复染的最适时间为40 s。甲基绿-派洛宁能够对细胞内的DNA和RNA同时进行定位、定性分析。该研究方法对实验教学、科学研究和临床实践具有一定的理论和实际意义。

Coloration observation of blood cell nucleic acids and spinal motor nerve cells in bullfrog

Objective To establish the coloration methods of blood cell nucleic acid and spinal motor nerve cells in bullfrog in order to better serve the teaching and clinic.Methods The temporary slides of bullfrog blood cells were prepared and the coloration of intracellular nucleic acid was performed by using the Feulgen reaction and methylgreen-pyronin.The bullfrog spinal motor nerve cells were stained by using toluidine blue.Results The nucleus was purple red after Feulgen reaction,and the cytoplasm was green.The optimum conditions for the reaction were hydrolysis for 2 min at the room temperature,for 8 min at 60℃,re-hydrolysis for 2 min at the room temperature,and re-dyeing for 40 s.After staining with methylgreen-pyronin,the nucleus appeared blue and the cytoplasm was pink.The spinal motor nerve cells were dyed dark blue.Conclusion The optimum time of light green re-dyeing in Feulgen reaction was 40 s.Methylgreen-pyronin is capable to simultaneously conduct the localization and qualitative analysis of intracellular DNA and RNA.This study method has a certain theoretical and practical significance for experimental teaching,scientific research and clinical practice.