不同离心条件对血清指数和生化免疫检测TAT的影响

目的 探讨不同离心条件组合对血清指数的影响,以及不同离心时间对生化免疫检测标本流转时间(TAT)的影响.方法 在50 d内,分别将离心条件设置为5种不同的组合:2000×g,10 min,15℃;2000×g,10 min,25℃;3000×g,10 min,15℃;3000×g,5 min,15℃;3000×g,7 ...

Influence of different centrifugal conditions on serum index and biochemical immunoassay TAT

Objective To investigate the effect of the combination of centrifugation conditions on the serum index and the effect of different centrifugation times on the turnover time(TAT)of biochemical immunoassay specimens.Methods Within 50 d,set the centrifugation conditions to 5 kinds different combinations,included 2000×g,10 min,15℃;2000×g,10 min,25℃;3000×g,10 min,15℃;3000×g,5 min,15℃;3000×g,7 min,25℃.Each group of centrifugal conditions was run for 10 d,and the influence of centrifugal force,centrifugation time,and centrifugal temperature on serum index were statistically analyzed;the influence of different centrifugal time on biochemical immunoassay TAT were counted.Results TAT was only affected by the centrifugation time.When the centrifugation time was set at 5 min from 14:00 to 16:00,TAT was the shortest in the laboratory.When the centrifugation time was set at 7 min from 12:00 to 14:00,TAT was the shortest in the laboratory.The hemolysis index were related to centrifugal time,centrifugal force,and centrifugal temperature.When the centrifugal force was 3000×g,the median hemolysis index was 5 mg/dL,and when the centrifugal force was 2000×g,the median hemolysis index was 4 mg/dL;when the centrifugation time was 10 min,the median hemolysis index was 5 mg/dL;when the centrifugation time was 5 min,the median hemolysis index was 4 mg/dL;when the centrifugation temperature was 25℃,the median hemolysis index was 3 mg/dL,and when the centrifugation temperature was 15℃,the median hemolysis index was 4 mg/dL.Conclusion Increasing the centrifugal force,prolonging the centrifugation time,and lowering the centrifugal temperature will increase the degree of hemolysis,but it is still within the clinically acceptable range.Based on the above factors,the best centrifugal conditions for this laboratory is 3000×g,7 min,25℃among the 5 combinations.