血清sCD14、HMGB1联合检测在肺部感染疾病中的临床诊断价值

目的探讨肺部感染患者血清可溶性白细胞分化抗原14(sCD14)、高迁移率族蛋白B1(HMGB1)临床应用价值。方法选取2017年10月至2018年6月在新疆军区总医院就诊的74例肺部感染患者作为实验组,同期62例健康体检者为对照组。采集研究对象血清标本,采用酶联免疫吸附测定法检测血清中sCD14、HMGB1水平;采用全自动免疫化学发光分析仪检测血清降钙素原(PCT)及白细胞介素-6(IL-6)水平。结果实验组sCD14、HMGB1水平分别为4.06(2.98~5.31)ng/mL、469.50(251.35~746.33)pg/mL,显著高于对照组(P<0.05)。单独sCD14、HMGB1检测诊断肺部感染的灵敏度依次为82.40%、56.80%,特异度为90.30%、85.50%,二者联合检测可提升灵敏度至92.40%,与PCT、IL-6联合检测的诊断效能接近。结论血清中sCD14、HMGB1联合检测对于肺部感染的辅助诊断具有一定价值,操作简捷、便携、易行,可作为缺少大型检验仪器设备的小、散、远医疗卫生机构快速诊疗肺部疾病的感染性标志物。

Clinical diagnostic value of combined detection of serum sCD14 and HMGB1 in pulmonary infection

Objective To investigate the clinical application value of serum soluble leukocyte differentiation antigen 14(sCD14)and high mobility group protein B1(HMGB1)in patients with pulmonary infection.Methods A total of 74 patients with pulmonary infections who were treated at the General Hospital of Xinjiang Military Region from October 2017 to June 2018 were selected as the experimental group,and 62 healthy persons who were examined during the same period were used as the control group.Serum specimens were collected,and the concentrations of sCD14 and HMGB1 in serum were measured by enzyme-linked immunosorbent assay.The levels of serum procalcitonin(PCT)and interleukin-6(IL-6)were measured by fully automatic immunochemiluminescence analyzer.Results The concentrations of sCD14 and HMGB1 in the experimental group were 4.06(2.98-5.31)ng/mL and 469.50(251.35-746.33)pg/mL respectively,which were significantly higher than those in the control group(P<0.05).The sensitivity of sCD14 and HMGB1 alone to diagnose lung infection was 82.40%,56.80%,and the specificity was 90.30%,85.50%,the combined detection could increase the sensitivity to 92.40%,which was close to the diagnostic efficiency of the combined detection with PCT and IL-6.Conclusion The combined detection of sCD14 and HMGB1 in serum is of certain value for the auxiliary diagnosis of lung infection,and it is simple,portable,and easy to use.It can be used as an infectious marker for the rapid diagnose and treatment of pulmonary infections in small,scattered and remote medical and health institution that lack large-scale inspection equipment.