2013-2015年血培养阳性标本污染情况分析

目的 分析探讨昆明市第一人民医院2013-2015年血培养污染率、污染途径、污染细菌构成比,为有效防止血培养污染提供依据.方法 对昆明市第一人民医院3年来共送检的34 713例血培养样本的污染情况进行回顾性分析.结果 血培养送检样本数34 713例,阳性培养样本数2 860例,总计污染样本数361例,污染率1.04%.按年度分类,2015年度血培养污染率最低;按季度分类,4个季度污染率差异不大;按内、外科两大系统分类,内科系统污染率1.06%,外科系统污染率0.96%;按科室分类,内科系统中污染率最高的科室是血透中心,污染率为2.71%,其次为重症医学科(ICU),污染率2.23%;外科系统中污染率最高的科室是乳腺科,污染率为2.30%,其次为骨科,污染率为1.92%;按污染菌菌株构成统计,人葡萄球菌污染居首位,占32.41%,其次为表皮葡萄球菌,占31.30%.结论 医院血培养污染率较高,污染途径多样化,血培养污染问题不容忽视,污染的细菌以血培养单瓶报阳的凝固酶阴性葡萄球菌为主.

Analysis of contamination of blood culture specimens from 2013 to 2015

Objective To analyze the rate of blood culture contamination,pollution path,the proportion of contaminated bacteria of First People′s Hospital of Kunming city from 2013 to 2015,and provide a basis for effective prevention and control of pollution.Methods A total of 34 713 cases of blood culture samples from 2013 to 2015,were retrospectively analyzed.Results A total of 2860 culture positive samples from 34 713 cases were found,from which 361 cases were polluted(1.04%).According to the classification of the year,the lowest rate of blood culture contamination was 2015 According to the classification in the quarter,there was no significant difference between the 4 seasons;According to the classification of internal and surgical systems,,the pollution rate of internal medicine system was 1.06%,while the surgical system was 0.96%.According to the classification of departments:the hemodialysis center has the highest pollution rate(2.71%),followed by ICU (2.23%).Galactophore Department has the highest pollution rate (2.30%) in the surgical system and followed by orthopedics(1.92%).According to the statistics of contaminated bacteria,Staphylococcus aureus was the highest,accounting for 32.41%,followed by Staphylococcus epidermidis,accounting for 31.30%.Conclusion Hospital has a high blood culture contamination rateand diversification pollution waywhich can not be ignored.The pollution of bacteria in the blood culture mainly for bottle of single positive coagulase negative Staphylococcus.