PCR-SSP与血清学技术用于ABO血型分型的比较

目的 探讨ABO血型基因分型技术应用于临床检验的可行性.方法 随机抽样100例江西健康汉族个体,并收集20例正反定型不符的标本,采用血清学和序列特异性引物PCR法(PCR-SSP)鉴定血型.结果 100例健康个体的基因分型结果与血清学分型结果一致,19例正反定型不符标本的基因分型结果准确.分型结果表明江西地区以O型为多,100例健康个体中,O型36例(36%),A型32例(32%),B型24例(24%),AB型8例(8%),基因频率:p(A)基因为0.225 3,q(B)基因为0.175 3,r(O)基因为0.599 8,且符合Hardy-Weinberg平衡.结论 基因分型技术应用于临床检验具有可行性,在鉴定疑难血型方面可以作为血清学分型的补充.

Comparison of PCR-SSP and serology technique for ABO blood group genotyping

Objective To discuss the possibility of applying ABO blood group genotyping by PCR-SSP in clinical.Methods 100 Han nationality people were extracted randomly,and 20 cases of samples with unconformity of positive and reverse ABO blood typing were collected at the same time.PCR-SSP and serology technique were applied to identify the blood grouping respectively.Results Blood grouping results of 100 ordinary cases detected by PCR-SSP and serology were coincident,and 19 common identification suspiciously cases’ genotyping results were accurately.Blood grouping results proved that blood group O was preponderance in Jiangxi.In 100 cases of healthy indivaduals,there were 36 cases of blood group O,32 cases of blood group A,24 cases of blood group B,and 8 cases of blood group AB,respectively.The gene distribution frequencies of p(A),q(B) and r(O) were 0.225 3,0.175 3,and 0.599 8 respectively,which were proved to be credible verified by Hardy-Weinberg Law.Conclusion The application of PCR-SSP in ABO blood group genotyping is viable in clinical,which could be a supplement to remedy the shortcomings of serology.