联用乳酸杆菌和丁酸梭菌对小鼠急性溃疡性结肠炎的影响

目的观察联用乳酸杆菌和丁酸梭菌对小鼠急性溃疡性结肠炎的疗效并探讨其治疗机制。方法建立DSS诱导的小鼠急性溃疡性结肠炎（UC）模型，观察给予乳酸杆菌和丁酸梭菌治疗后，小鼠结肠黏膜的病理改变和TNF—α和组织因子（TF）表达的变化。结果乳酸杆菌和丁酸梭菌可明显减轻小鼠结肠黏膜的损伤；可明显抑制TNF—α和TF的表达，尤以两菌合用组抑制作用最强。结论乳酸杆菌和丁酸梭菌对DSS诱导的UC有治疗作用，对TNF-α和TF表达的协同抑制作用可能足其发挥协调治疗作用的分子机制。

Influence of combined administration of lactobacilli and clostridium butyricum on acute mice ulcerative colitis

Objective To investigate the therapeutic effect of combined administration of lactobacilli and clostridium butyricum on acute mice ulcerative colitis, and explore its therapeutic mechanism.Methods Dextran sulfate sodium (DSS)-induced mice model of acute ulcerative colitis was established. After administration of lactobacilli and clostridium butyricum, the pathological change of tunica mucosa coli was observed and the expression levels of tumor necorisis factor (TNF-α) and tissue factor (TF) were measured.Results Lactobacilli and clostridium butyricum significantly alleviated the damage of tunica mucosa coli and suppressed the expression of TNF-αand TF. By comparison, there were the lightest histological damage and the lowest expression of TNF-αand TF when lactobacilli and clostridium butyricum were administrated combined.Conclusion Both lactobacilli and clostridium butyricum show therapeutic effect on DSS-induced mice ulcerative colitis. The coordinate repression on expression of TNF-αand TF may be the molecular mechanism of the co-effect on mice UC.