ROS对糖尿病大鼠血脂代谢及肾脏组织中VEGF的影响

目的探讨地黄寡糖(ROS)对糖尿病大鼠血脂代谢及肾脏组织中血管内皮生长因子(VEGF)的影响。方法将40只SPF级大鼠按照随机数字表法分为健康组、DM组、ROS组及药物对照组,每组10只。DM组、ROS组及药物对照组大鼠均建立糖尿病模型。ROS组及药物对照组分别采用200 mg/(kg·d)的ROS及二甲双胍灌胃,剩余两组灌胃等体积生理盐水,治疗22 d。治疗结束后,检测血脂代谢指标、肾脏重量、肾脏病理组织切片、肾脏组织中VEGF阳性率及蛋白。结果与健康组比较,DM组、ROS组、药物对照组总胆固醇(TC)、三酰甘油(TG)升高,高密度脂蛋白胆固醇(HDL-C)降低,经药物干预后,ROS组、药物对照组TC、TG明显低于DM组,HDL-C明显高于DM组,且ROS组TG明显低于药物对照组,差异有统计学意义(P<0.05)。与健康组比较,ROS组、药物对照组大鼠肾脏重量及肾脏指数均明显增加,药物干预后,与DM组比较,ROS组及药物对照组肾脏重量及肾脏指数明显降低,差异有统计学意义(P<0.05)。健康组大鼠肾脏结构完整,肾小球基质分布清晰,未见增生及肥大;DM组大鼠肾小球基质模糊,毛细血管增厚,炎性反应浸润严重,肾小球出现肥大;经药物干预的ROS组及药物对照组上述病理情况均好转,症状减轻。健康组、DM组、ROS组及药物对照组大鼠肾脏组织中VEGF蛋白相对表达量分别为1.00±0.15、3.03±0.33、1.45±0.27、1.50±0.30,组间比较,差异有统计学意义(F=109.100,P<0.001)。DM组VEGF蛋白相对表达量明显高于健康组,差异有统计学意义(t=17.880,P<0.001)。ROS组及药物对照组VEGF蛋白相对表达量明显低于DM组,差异有统计学意义(t=11.870、10.990,P<0.001)。结论灌胃ROS能够改善糖尿病大鼠血脂代谢指标,降低肾脏重量,这与抑制VEGF表达具有相关性。

Effect of ROS on blood lipid metabolism and VEGF level in kidney tissue of diabetic rats

Objective To explore the effect of rehmannia glutinosa oligosaccharides(ROS)on blood lipid metabolism and vascular endothelial growth factor(VEGF)level in kidney tissue of diabetic rats.Methods A total of 40 SPF rats were divided into healthy group,DM group,ROS group and drug control group,ten rats in each group.Diabetic models were established in DM group,ROS group and drug control group.ROS group and drug control group were given 200 mg/(kg·d)ROS and metformin respectively,the remaining two groups were given the same volume of saline,treatment for 22 d.Results The total cholesterol(TC),triacylglycerol(TG)in DM group,ROS group and drug control group increased,high density lipoprotein cholesterol(HDL-C)decreased compared with those in healthy group.After drug intervention,the TC,TG in ROS group and drug control group were significantly lower than those in DM group,HDL-C was significantly higher than that in DM group,and the TG in ROS group was significantly lower than that of drug control group(P<0.05).The kidney weight and kidney index of rats in ROS group and drug control group significantly increased compared with those in healthy group.After drug intervention,the kidney weight and kidney index of ROS group and drug control group significantly decreased compared with those in DM group(P<0.05).The kidney of rats in healthy group was intact,the distribution of glomerular matrix was clear,no hyperplasia or hypertrophy.Glomerular matrix blur in DM group,capillary thickening,infiltration of inflammatory response,glomerular hypertrophy.Both the ROS group and the drug intervention group improved,symptoms abated.The relative expression of VEGF proteins in kidney tissues of healthy group,DM group,ROS group and drug control group were 1.00±0.15,3.03±0.33,1.45±0.27,1.50±0.30,the differences were statistically significant(F=109.100,P<0.001).The relative expression of VEGF protein in DM group was significantly higher than that in healthy group(t=17.880,P<0.001).The relative expression of VEGF protein in ROS group and drug control group were significantly lower than that in DM group(t=11.870,10.990,P<0.001).Conclusion Gavage of ROS could improve the index of blood lipid metabolism and reduce the weight of kidney in diabetic rats,which might relate to the inhibition of VEGF expression.