乙型肝炎患者HBV DNA定量与血清标志物的关系分析

目的分析乙型肝炎病毒（HBV）血清标志物与HBV DNA定量之间的关系。方法分别采用酶联免疫吸附试验（ELISA）和荧光定量PCR（FQ-PCR）对391例乙型肝炎表面抗原（HBsAg）阳性血清进行检测，分析检出率及相关性。结果391例HBsAg阳性血清共检出7种模式，以HBV DNA含量大于5×10^2copy／mL为阳性。HBsAg阳性（＋）、乙型肝炎e抗原（HBeAg）（＋）、乙型肝炎核心抗体（抗-HBc）（＋）模式的阳性检出率较高，为96．9％，HBV DNA含量也较高，其中大于10^7copy／mL的血清为53．8％。HBsAg（＋）、抗-HBe（＋）、抗-HBc（＋）模式中阳性检出率为25．3％，171例（74．7％）HBV DNA含量小于5×10^2copy／mL。HBsAg（＋）、抗-HBc（＋）模式中阳性检出率为65．4％。结论HBsAg和HBV DNA联合检测，更能反映乙型肝炎患者HBV感染、传染性及体内复制情况。

The relationship between the detection of HBV markers and HBV DNA

Objective To explore the relationship between the serum contents of HBV DNA and the HBV serological markers. Methods The content of HBV DNA copy was detected by fluorescence quantitative PCR（FQ- PCR）,and the HBV serum index of immunology by ELISA in 391 samples. Results Seven modes were dectected from the 391 samples with HBsAg positive. HBV DNA level〉5 ×10^2 copy/mL is positive. In the mode with HBsAg＋, HBeAg＋ ,HBcAb＋ ,the positive rate is 96.9% ,and HBV DNA level is also high（〉10^7 copy/mL,53.8%）. In the following group with HBsAg＋, HBeAb＋, HBcAb＋ ,the positive rate is 25.3%, the serum contents of HBV DNA〈5× 10^2 copy/mL in the other 171 samples. In the mode with HBsAg＋, HBcAb＋,the positive data was 65.4%. Conclusion It is significant to combine the HBV serological markers with HBV DNA level,which can better reflect the real conditions of HBV infection and replication in the patients with HBV.