1659株伤口分泌物中分离病原菌种类及耐药性分析

目的了解伤口分泌物中分离病原菌种类及耐药性,为合理用药提供依据。方法细菌鉴定和药敏试验主要采用Phoenix100分析仪,念珠菌鉴定采用显色平板法,药敏试验采用K-B法。数据分析采用WHO-NET5.6软件。结果伤口分泌物中分离病原菌中最常见的为金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌、粪肠球菌、表皮葡萄球菌、肺炎克雷伯菌和鲍曼不动杆菌。大肠埃希菌和肺炎克雷伯菌产超广谱β内酰胺酶菌株所占比例分别为62.8%和52.4%。革兰阴性杆菌中耐药率较低的为亚胺培南、美洛培南、阿米卡星、头孢哌酮/舒巴坦、哌拉西林/他唑巴坦和头孢他啶。金黄色葡萄球菌、表皮葡萄球菌和溶血葡萄球菌甲氧西林耐药率分别为34.2%、87.7%和90.6%。革兰阳性球菌对万古霉素、替考拉宁和利奈唑胺的敏感率均为100.0%。念珠菌对临床常用抗菌药物的耐药率均低于10.0%。结论本院伤口分泌物中分离病原菌耐药率较高,应合理使用抗菌药物以降低耐药率。

Distribution and antibiotic resistance analysis of 1 659 pathogen isolated from wound secretion

Objective To investigate distribution and drug resistance of bacteria and fungi isolated from wound secretion for using antibiotic reasonably. Methods Most bacteria isolates were identified with Phoenix100 analyzer. Candida isolates were identified by color display plate and by K-B method. WHONET5.6 was applied for data analy- sis. Results The common isolates were S. aureu,E, coli,P, aeruginosa,E, faecalis,S, epidermidis,K, pneumoniae,and A. baumannii. The incidences of E. coli and K. pneumoniae isolates producing extended spectrum beta-lactamase were 62.8% and 52.40/oo respectively. Resistant rate of gram negative bacillus to Imipenem, Meropenem,Amikacin,Cef- operazone/Sulbactam,Piperacillin/Tazobactam,and Ceftazidime were lower. The meticillin resistant rates of S. aureu, S. epidermidis,and S. haemolyticus were 34.2% .87.7% and 90.6% respectively. In gram positive coccus,susceptible rates to Vancomycin,Teicoplanin and Linezolid were 100. 0%. In Candida isolates, resistant rates to common drugs were below 10. 0%. Conclusion Resistant rates of pathogen isolated from wound secretion in this hospital were high. It is important to enforce the rational use of antimicrobial agents to reduce resistant rates of bacteria.