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血中杜冷丁亲水性材料固相提取法
引用本文:吴玉红,王勇,王雪琴,卜娟,张奕奕.血中杜冷丁亲水性材料固相提取法[J].检验医学与临床,2016(Z2):19-20.
作者姓名:吴玉红  王勇  王雪琴  卜娟  张奕奕
作者单位:重庆警察学院刑事科学技术系,重庆,401331
基金项目:重庆市高校优秀成果转化项目“药物毒物分析中提取关键技术的成果转化”资助(KJZH14109)。
摘    要:目的:建立血中杜冷丁的亲水性固相材料提取方法。方法取1.0mL检血加1.0mL水稀释,倒入装有2.3g亲水性固相材料小柱中,用10 mL二氯甲烷或乙醚洗脱,洗脱液中加利多卡因,挥干,用乙酸乙酯定容至0.1mL。结果二氯甲烷提取率(mean ± SD)为(95.1±3.2)%,检出限13ng/mL ;乙醚提取率(mean ± SD)为(74.4±3.1)%。回收率100.8%,线性范围1.0~40μg/m L。结论该法简便、快速,适合作为常规毒物分析方法。

关 键 词:亲水性固相材料  杜冷丁  气相色谱法

The blood of meperidine hydrophilic material of solid-phase extraction
Abstract:Objective Extraction method of hydrophilic solid material to establish the blood of meperidine .Methods Take 1 .0 mL blood plus 1 .0 mL diluted in water ,poured into the hydrophilic solid material with 2 .3g column ,eluted with dichloromethane or diethyl ether 10mL ,eluent and lidocaine ,evaporating ,using ethyl acetate volume to 0 .1mL .Results The extraction rate of dichlo-romethane (mean + SD) for (95 .1 + 3 .2)% ,the detection limit of 13ng/mL ;ether extraction rate (mean + SD) for (74 .4 + 3 . 1)% .Recovery rate of 100 .8% ,linear range of 1 to 40 g/mL .Conclusion The method is simple and rapid ,and it is suitable for the analysis of conventional poison .
Keywords:Hydrophilic solid material  Pethidine  Gas chromatography
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