血中杜冷丁亲水性材料固相提取法 |
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引用本文: | 吴玉红,王勇,王雪琴,卜娟,张奕奕.血中杜冷丁亲水性材料固相提取法[J].检验医学与临床,2016(Z2):19-20. |
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作者姓名: | 吴玉红 王勇 王雪琴 卜娟 张奕奕 |
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作者单位: | 重庆警察学院刑事科学技术系,重庆,401331 |
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基金项目: | 重庆市高校优秀成果转化项目“药物毒物分析中提取关键技术的成果转化”资助(KJZH14109)。 |
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摘 要: | 目的:建立血中杜冷丁的亲水性固相材料提取方法。方法取1.0mL检血加1.0mL水稀释,倒入装有2.3g亲水性固相材料小柱中,用10 mL二氯甲烷或乙醚洗脱,洗脱液中加利多卡因,挥干,用乙酸乙酯定容至0.1mL。结果二氯甲烷提取率(mean ± SD)为(95.1±3.2)%,检出限13ng/mL ;乙醚提取率(mean ± SD)为(74.4±3.1)%。回收率100.8%,线性范围1.0~40μg/m L。结论该法简便、快速,适合作为常规毒物分析方法。
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关 键 词: | 亲水性固相材料 杜冷丁 气相色谱法 |
The blood of meperidine hydrophilic material of solid-phase extraction |
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Abstract: | Objective Extraction method of hydrophilic solid material to establish the blood of meperidine .Methods Take 1 .0 mL blood plus 1 .0 mL diluted in water ,poured into the hydrophilic solid material with 2 .3g column ,eluted with dichloromethane or diethyl ether 10mL ,eluent and lidocaine ,evaporating ,using ethyl acetate volume to 0 .1mL .Results The extraction rate of dichlo-romethane (mean + SD) for (95 .1 + 3 .2)% ,the detection limit of 13ng/mL ;ether extraction rate (mean + SD) for (74 .4 + 3 . 1)% .Recovery rate of 100 .8% ,linear range of 1 to 40 g/mL .Conclusion The method is simple and rapid ,and it is suitable for the analysis of conventional poison . |
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Keywords: | Hydrophilic solid material Pethidine Gas chromatography |
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