乙型肝炎病毒DNA定量与血清学标志物的关系

目的探讨乙型肝炎（下称乙肝）病毒（HBV）DNA定量与HBV血清学标志物（HBVM）的关系。方法采用实时荧光定量聚合酶链反应（FQ-PCR）检测225例乙肝感染者血清的HBV DNA含量，并用酶联免疫吸附试验（ELISA）对其血清学标志物进行检测。结果在不同HBV M模式中，HBV DNA与HBV前S1抗原（preS1Ag）总检出率差异无统计学意义。在模式乙肝病毒表面抗原（HBsAg）（＋）、乙肝病毒e抗原（HBeAg）（＋）和抗核心抗原抗体（＋）中血清HBV DNA含量明显高于其他模式。在139例HBV DNA阳性的标本中，HBV DNA与preS1Ag检出率差异无统计学意义（P〉0．05），HBV DNA与HBeAg检出率差异有统计学意义（P〈0．01），同时preS1Ag阳性组HBV DNA定量值显著高于preS1Ag阴性组。结论HBV DNA或preS1Ag与HBV复制密切相关，preS1Ag较HBeAg更能敏感反应HBV复制，联合检测HBV DNA与HBVM在乙肝的诊断治疗中更有重要的临床指导价值。

Relationship between the quantitation of HBV DNA and HBV markers

Objective To explore the relationship between the quantitation of HBV DNA and HBV markers （HBV M）. Methods HBV DNA levels were measured in 225 cases of serum samples from hepatitis B patients with real time fluorescent quantitative PCR （FQ-PCR）,and HBV M were also detected by applying ELISA. Results In different modes of HBV M, the difference wasn＇t statistically significant in total detection rate of serum HBV DNA and preS1 antigen. The serum HBV DNA level was the highest in mode of HBsAg （＋）HBeAg （＋）HBcAb （＋）. In 139 serum samples with HBV DNA posilive, the difference wasn＇t statistically significant in the detection rate of serum HBV DNA and preS1 antigen （P〉0. 05）. In contrast, there was significant difference between the detection rates of serum HBV DNA and HBeAg （P〈0.01）. and while HBV DNA level was more elevated in preS1 positive group than that of preS1 negativc group. Conclusion HBV DNA or preS1 closely correlates with hepatitis B virus replication. It shows that preS1 is more sensitive than HBeAg in reflecting hepatitis B virus replication. Combined detection of HBV DNA and HBV M contributes to the diagnosis and treatment of hepatitis B.