清热解毒方对可移植性小鼠微小残留白血病实验研究

目的探讨清热解毒方对微小残留白血病模型的疗效、作用机制,并就其对2种不同肿瘤负荷的疗效进行比较。方法造模成功后予高剂量清热解毒方灌胃7d,观测生存时间、外周血白细胞计数及分类、骨髓有核细胞涂片分类、肝脾指数、肝脾骨髓病理形态、残留白血病细胞、细胞增殖周期及细胞凋亡。结果清热解毒方可延长微小残留白血病小鼠的生存时间(P<0.05),且肿瘤负荷较小时疗效较好。结论清热解毒方对微小残留白血病有效。减轻白血病细胞对脏器的浸润、抑制MRL小鼠骨髓细胞的增殖活力、促进细胞凋亡,这可能是该方部分作用机制。

Experimental Study on Effect of Qing-re-jie-du Recipe on Minimal Residual Leukemia Model Mice

Objective: To evaluate the therapeutic effect of Qingre Jiedu Recipe (QRJDR ) on minimal residual leukemia ( MRL ) model?explore its mechanism and compare the effect of QRJDR on two different tumor cell burden of the model.Methods: the established model mice were treated with high dosage of oral QRJDR for 7 days. Then their survival time?leukocyte count in peripheal blood and bone marrow?liver and spleen index?pathological study of the liver?spleen and bone marrow?residual leukemic cells?cell cycle and poptosis were observed. Results: QRJDR could prolong the survival time of model mice ( P <0.05) and the effect was better when tumor cell burden was lower.Conclusion: QRJDR was effective on MRL and the effect was better when tumor cell burden was lower. QRJDR could lower the organ leukocyte infiltration?inhibit the proliferation of bone marrow cells of model mice and induce apoptosis which might be partial mechanism of QRJDR.