洗腿又方治疗骨关节炎的网络药理学研究

目的 通过生物信息学、网络药理学和分子对接探讨洗腿又方治疗骨关节炎的药理学机制。方法 从基因表达综合数据库(GEO)中获取骨关节炎滑膜的差异表达基因，使用TCMSP、HERB、TCMID等数据库搜集洗腿又方的活性成分及其作用靶点，并与疾病靶点取交集。通过STRING数据库及Cytoscape3.8.2软件对交集靶点进行蛋白互作和富集分析，最终将关键活性成分与关键靶点进行分子对接。结果 获得骨关节炎滑膜的差异表达基因2072个，洗腿又方的活性成分20种，其治疗骨关节炎的靶点30个，进一步通过蛋白互作筛选获得HIF1A、CASP8、RAF1等关键靶点，富集分析发现洗腿又方主要通过干预细胞周期、炎症和内分泌等信号通路治疗骨关节炎，分子对接结果显示，关键有效成分芹黄素、槲皮素分别与关键靶点HIF1A、CASP8有良好的对接活性。结论 本研究探讨了洗腿又方治疗骨关节炎的可能活性成分、靶点及作用通路，为其应用于骨关节炎的治疗提供了理论依据。

Network pharmacological research of Xituiyoufang in the treatment of osteoarthritis

Objective To explore the pharmacological mechanism of Xituiyoufang in the treatment of osteoarthritis through bioinformatics, network pharmacology and molecular docking. Methods We obtained differentially expressed genes in the synovial membrane of osteoarthritis from gene expression omnibus(GEO).We combined with relevant literature and collected the active components and target of Xituiyoufang, and got the intersection with the disease target by using TCMSP, HERB, TCMID and other databases.Through the STRING database and Cytoscape3.8.2 software, the protein interaction and enrichment analysis of the intersection targets were carried out, and the key active components were finally molecularly docked with the key targets. Results There were 2072 differentially expressed genes in the synovial membrane of osteoarthritis, 20 active ingredients of Xituiyoufang, and 30 targets for the treatment of osteoarthritis. Key targets such as HIF1A, CASP8, and RAF1 were further obtained through protein interaction screening.Enrichment analysis found that Xituiyoufang mainly treats osteoarthritis by intervening cell cycle, inflammation and endocrine signaling pathways. The molecular docking results show that the key active ingredients Baicalein and Quercetin are respectively associated with the key target HIF1A. , CASP8 has good docking activity. Conclusion This study explored the possible active components, targets and action pathways of Xituiyoufang in the treatment of osteoarthritis, and provided a theoretical basis for its application in the treatment of osteoarthritis.