急性髓系白血病细胞外基质纤连蛋白、基膜连接蛋白的分析及临床意义

目的探讨细胞外基质纤连蛋白（Ｆｎ）、基膜连接蛋白（Ｌｍ）与白血病细胞生长、分化之间的关系。方法应用免疫荧光法观察１８例急性髓细胞白血病（ＡＭＬ）骨髓细胞长期培养（ＬＴＢＭＣ）细胞外基质Ｆｎ、Ｌｍ含量的变化。结果①９例获独立生长的ＡＭＬ（ＡＭＬｓｍ）骨髓细胞在培养的１～３周，Ｆｎ、Ｌｍ含量均显著低于９例未获独立生长的ＡＭＬ（ＡＭＬｎｓｍ）；②在培养的第３周，８例ＡＭＬｎｓｍ幼稚细胞比例可降至０．０５以下，而７例ＡＭＬｓｍ未能降至０．０５；③各周幼稚细胞比例分别与Ｆｎ、Ｌｍ含量相关分析示：第０～８周正常对照均呈正相关，０～４周ＡＭＬｎｓｍ均呈负相关而ＡＭＬｓｍ仅Ｆｎ组呈负相关。结论Ｆｎ和（或）Ｌｍ的改变在ＡＭＬ发生、发展的某一环节起重要作用。此外，Ｆｎ、Ｌｍ含量在ＡＭＬｓｍ与ＡＭＬｎｓｍ之间不同，这对ＬＴＢＭＣ应用于ＡＭＬ自身骨髓体外净化病例的选择可能有参考价值。

Analysis of fibronectin and laminin in the extracellular matrixes of acute myeloid leukemia and its implications

OBJECTIVE: To investigate the relationship between quantitative change of fibronectin (Fn) or Laminin (Lm) and growth and differentiation of leukemic cells. METHODS: The extracellular matrixes, Fn and Lm, in long-term bone marrow culture (LTBMC) from 18 acute myeloid leukemia (AML) patients and 6 normal subjects were examined by immunofluorescence assay. RESULTS: 1. The Fn or Lm content in 9 AML self-maintained (AMLsm) 1-3 week-cultures was significantly less than that in 9 AML not self-maintained (AMLnsm) cultures. 2. In the third week's cultures, the blasts of 8 AMLnsms decreased to less than 5%, but did not of 7 AMLsms. 3. As the cultures going on, the blasts were related to Fn or Lm contents positively in normal control and negatively in AMLnsm. The blasts were related to Fn contents negatively in AMLsm. CONCLUSION: The change of Fn or Lm may play an important role in the development of AML. In addition, the difference of Fn or Lm contents between AMLsm and AMLnsm was helpful to the choice of AML patient for in vitro purging of leukemic cells through LTBMC before auto-BMT.