ω-3不饱和脂肪酸和谷氨酰胺抗压力性氧化损伤的实验研究

目的研究（ω-3不饱和脂肪酸（polyunsaturated fattyacids，ω-3 PUFAs）和谷氨酰胺（glutalnin，Gin）对老年大鼠受压组织细胞的抗氧化损伤作用，并探讨其可能的机制。方法将SPF级雄性老年SD大鼠28只按随机数字表法分成对照组、模型组、ω-3 PUFAs干预组（A组）、Gln干预组（B组）。A、B组大鼠分别给予PUFAs及Gln配制液灌胃喂养20d；对照组不受压，模型组及A、B组早期压疮模型诱导成功后，4组大鼠一起实施安乐死。观察受压组织病理形态学变化；检测各组乳酸脱氢酶（lactate dehydrogenase，LDH）漏出液、10％肌肉匀浆液中超氧化物歧化酶（superoxide dismutase，SOD）的活性及一氧化氮（nitricoxide，NO）含量的变化。结果受压组织病理形态学显示，与模型组相比，A组受压组织受损程度明显减轻（P〈0．01），B组受压组织受损程度有所好转（P〈0．05）。生化检测显示，A组受压组织SOD活性显著增高、LDH漏出液和NO含量显著减少（均P〈0．01）；B组受压组织SOD活性增高、LDH漏出液减少（均P〈O．05），NO含量显著降低（P〈0．01）。结论ω-3 PUFAs和Gln早期干预均能提高受压组织抗氧化能力，尤其是ω-3 PUFAs对老年大鼠抗压力性氧化损伤作用较强。

Effect of ω-3 Unsaturated Fatty Acids and Glutamine Against Resistance Oxidative Damage

Objective To study the effect against oxidative damage of ω-3 unsaturated fatty acid （ω-3PUFAs） and glutamine （Gln） on compression tissue cell of elderly rats,and explore its possible mechanisms. Methods Twenty eight SPF-level elderly male SD rats were randomly assigned to four groups, which were the control group, the model group, the ω-3PUFAs intervention group （group A） and the Gln intervention group （group B）, respectively. The rats in group A were fed by lavaging on the ω-3PUFAs configuration liquid and rats in group B were fed by lavaging on the Gln configuration liquid. All of the rats in the four groups were euthanized after the model success- fully built, where the control group were given no pressure while the model group, group A and the group B were induced early bedsore model. Based on that, we observed the pathological morphology change of the pressure tissue,tested the content change of the LDH leakage liquid and 10% muscle homogenate super Oxide dismutase （SOD） activity and nitric oxide （NO）. Results Compared with the model group, the level of the compression tissue in the group A and group B were highly significantly reduced （P〈0.01,P〈0.05）. Biochemical test showed that SOD activity of compression tissue in group A was significantly increased while LDH leakage liquid and NO content was significantly reduced （P〈0.01） ; SOD activity of compression tissue in group B increased while LDH leakage liquid reduced （P〈0. 05） and NO content significantly reduced （P〈0.01）. Conclusion Both the ω-3PUFAs and Gln early intervention can improve compression tissue antioxidant ability, especially the ω-3PUFAs,whose function of compressive resistance in aged rats was strong.