| 流式细胞术计数CD34阳性细胞的标准化与质量控制 |
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| 引用本文： | 刘艳荣,陈珊珊,于弘.流式细胞术计数CD34阳性细胞的标准化与质量控制[J].中国实验血液学杂志,2000,8(4):302-306. |
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| 作者姓名： | 刘艳荣 陈珊珊 于弘 |
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| 作者单位： | 北京大学血液病研究所,北京,100044 |
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| 摘 要： | 用流式细胞术计数脐带血，外周血及其采集物中的ＣＤ３４＾＋细胞数已被普遍采用，然而，不同实验之间的ＣＤ３４＾＋细胞计数差异非常大，本就影响结果的主要因素，目前国际上采用的标准化方案和不同方案之间的比较进行综述，我们实验室应用ＰｒｏＣＯＵＮＴ试剂盒检测了４５例脐带血，１２份正常骨髓和４例外周血采集物中的ＣＤ３４＾＋细胞的数量，结果表明ＰｒｏＣＯＵＮＴ为标准化程度较高的方法，有利于减少不同实验之间ＣＤ３４＾＋细胞计数的差异。
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| 关 键 词： | CD34细胞 流式细胞术 标准化 质量控制 |
| 修稿时间： | 2000年4月2日 |
Standardization and Quality Control in Flow Cytometric Enumeration of CD34+ cells |
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| LIU Yanrong,CHEN Shanshan,YU Hong.Standardization and Quality Control in Flow Cytometric Enumeration of CD34+ cells[J].Journal of Experimental Hematology,2000,8(4):302-306. |
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| Authors: | LIU Yanrong CHEN Shanshan YU Hong |
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| Abstract: | The enumeration of CD34 cells by flow cytometry is commonly employed to assess hematopoietic stem/progenitor cells in cord blood, peripheral blood and apheresis products. Interlaboratory variation of CD34 cells enumeration is exceedingly large. Factors contributing to those variation, currently established flow cytometry protocols and comparison between these protocols were reviewed. CD34 cells in 45 cord blood samples, 12 normal bone marrows and 4 apheresis products were also enumerated in our laboratory by using ProCUNT kit, and results showed that the ProCUNT is highly standardizaed and could assist in reducing interlaboratory variation of CD34 cells. |
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| Keywords: | CD34 cell enumeration flow cytometry standarizaion quality control |
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