C反应蛋白对多发性骨髓瘤细胞系U266增殖机制的研究

为了观察C反应蛋白（CRP）对人骨髓瘤细胞系U266细胞增殖活性的作用机制，将液氮冻存的U266细胞复苏后，悬浮培养，第3天收集细胞备用，分别以不同浓度CRP（0、5、10、20mg／L）培养24小时，采用血液分析仪检测细胞增殖情况，采用逆转录-聚合酶链反应（RT—PCR）方法检测凋亡相关蛋白survivin和HSP90ct的表达。结果发现：CRP处理的细胞组增殖率及survivin、HSP90α的表达量均高于对照组（P〈0．05），20mg／LCRP组作用最显著；survivin、HSP90α之间表达具有显著相关性（r=0．737，P〈0．0001）。结论：CRP是通过调节凋亡抑制蛋白Survivin及HSP90α的表达发挥促进U266细胞增殖的作用。

Mechanism of C-Reactive Protein on Proliferation of Multiple Myeloma U266 Cells

This study was purposed to investigate the mechanism of C-reactive protein （CRP） on proliferation of U266 cells. The human multiple meyloma cell line U266 was incubated with human CRP（0,5,10,20 mg/L） for 24 hours, then the proliferation level of U266 cells was detected by using blood analyser. The mRNA expressions of survivin and HSP90α were examined by RT-PCR. The results showed that the proliferation ratio was increased, as compared with the control group（p 〈0.05 ） ; furthermore, the mRNA levels of survivin and HSP90α were up-regulated in proportion to the increased CRP concentrations. There was significant correlation between expression of survivin and HSP90et （ r = 0. 737 ,p 〈 0.0001 ） in incubated cells. It is concluded that CRP can stimulate the proliferation of MM cells directly by up-regulating the expression of survivin and HSP90α in MM cells. CRP can be regarded as a potential target for MM treatment.