急性髓系白血病初诊患者β-连环蛋白和周期蛋白D1mRNA的表达及其意义

本研究旨在检测急性髓系白血病（AML）患者β-连环蛋白（beta—catenin）及周期蛋白D1（cyclinD1）mRNA的表达变化，分析其相关性，为探讨Wnt／β联蛋白（Wnt／beta—catenin）通路在AML发病机制中的作用提供理论依据。采用实时荧光定量RT—PCR的方法检测beta—catenin及cyclin D1 mRNA的相对表达水平，分析两者在AML不同亚型及良性血液病患者中的表达变化及相关性。结果表明，AML骨髓单个核细胞（BMMNC）中beta-cateninmRNA的表达水平明显高于良性血液病患者（P〈0．05），但在AML各亚型之间其表达量无统计学差异（P〉0．05，在AML中存在cyclinD1 mRNA的过度表达，其表达水平明显高于良性血液病组（P〈0．05），在各亚型间其表达量无统计学差异（P〉0．05）。Beta-catenin与cyclinD1的表达水平在AML-M1、M2、M4中存在显著正相关性（r值分别为0．822，0．627，0．712，P值分别为0．001，0．020，0．002）。结论：在AML患者中存在beta—catenin及cyclin D1的过度表达，且在部分亚型中存在显著相关性；Wnt／beta-catenin通路在AML中被异常激活，很可能是通过该通路下游靶基因cyclinD1的激活，参与白血病细胞的周期紊乱和异常增殖的调节。

Beta-catenin and Cyclin D1 mRNA Levels in Newly Diagnosed Patients with Acute Myeloid Leukemia and Their Significance

This study was aimed to quantitatively detect the levels of beta-catenin and cyclin D1 mRNA in various subgroups of acute myeloid leukemia （AML） and to analyze their potential relationship, so as to provide theoretical basis for exploring the role of Wnt/beta-catenin pathway in the pathogenesis of AML. Real time fluorescent quantitative RT- PCR was used to detect the relative expression levels of beta-catenin and cyclin D1 mRNA, to analyze changes of the two gene expressions and their relationship. The results showed that the beta-catenin mRNA expression level in BMMNC of AML patients was significantly higher than that in benign blood disease patients （p 〈 0.05）, but no statistical difference was found among the various subgroups of AML（p 〉 0.05 ）. In AML there was overexpression of cyclin D1 mRNA, and its expression level was significantly higher than that in benign blood disease group（p 〈 0.05 ）, but there was no statistical difference among the subtypes of AML. The expression levels of beta-catenin and cyclin D1 were correlated each other in AML-M1 , M2 and M4 （ r values were 0. 822,0. 627,0. 712 respectively; p values were 0.001, 0.020, 0.002 respectively ）. It is concluded that the over-expressions of beta-cateniu and cyclin DI exit in AML patients, and the significant correlation appears in part of the subgroups, which means that the Wnt/beta-catenin pathway is aberrantly activated in AML, probably activating the downstream target gene cyclin D1 and participating in the regulation of cell cycle disturbance and abnormal proliferation of leukemic cells.