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四色荧光标记抗体在白血病免疫分型中的应用及意义
引用本文:刘艳荣,于弘,常艳,陈珊珊.四色荧光标记抗体在白血病免疫分型中的应用及意义[J].中国实验血液学杂志,2002,10(5):423-427.
作者姓名:刘艳荣  于弘  常艳  陈珊珊
作者单位:北京大学血液病研究所,北京,100044
摘    要:为探讨多参数流式细胞术对白血病免疫分型的方法及意义,本研究自行设计了一系列4种荧光标记的单克隆抗体组合,对88例急性白血病患骨髓或外周血进行流式细胞仅免疫表型分析。应用本组抗体组合可对T—ALL和B—ALL及AML-M1—M7患做出诊断,并可诊断形态学难以辨认的AML-M0,及一般免疫分型法较难辨认的单核细胞白血病。应用4组四色荧光标记的抗体组合,共含13种抗体,既可将90%以上的白血病患分为ALL或AML,并可鉴别急性白血病的主要亚型,及反映血细胞的分化成熟度。结论:四色荧光抗体组合可用较少的抗体,特异地对白血病细胞进行多参数免疫分型分析,提高免疫分型的准确性。

关 键 词:白血病  免疫分型  四色荧光标记抗体  多参数分析  多参数流式细胞术
修稿时间:2002年3月13日

The Application of 4-Color Fluorescence Labeling Antibodies and Its Significance in Immunophenotyping for Leukemia by Flow Cytometer
LIU Yan Rong,YU Hong,CHANG Yan,CHEN Shan Shan.The Application of 4-Color Fluorescence Labeling Antibodies and Its Significance in Immunophenotyping for Leukemia by Flow Cytometer[J].Journal of Experimental Hematology,2002,10(5):423-427.
Authors:LIU Yan Rong  YU Hong  CHANG Yan  CHEN Shan Shan
Institution:Institute of Hematology, Peking University, Beijing 100044, China.
Abstract:To prove the significance of 4 color fluorescence labeling flow cytometric analysis for leukemia immunophenotyping, bone marrow and peripheral blood samples from 88 cases with acute leukemia were analyzed by means of flow cytometry with 4 color fluorescence labeling monoclonal antibodies(McAbs). More than 90% of cases could be classified into acute myeloid, T or B lymphocytic leukemia by staining with 13 different kinds of monoclonal antibodies within 4 tubes of combination of antibodies. AML M 2-M 7 could also be identified simultaneously. Another one tube of combination of 4 McAbs could be used for further subtyping of those who were undetermined by the above 4 tubes. Patients with AML M 0/M 1 which may be negative of myeloperoxidase(MPO) in cytochemistry can be identified by labeling with both membrane and cytoplasmic antibodies. In conclusion, the accuracy of leukemia immunphenotyping has been improved by 4 color flow cytometry analysis.
Keywords:leukemia  immunophenotyping  4  color fluorescence labeling antibdy  multiparameter flow cytometry
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