Sysmex CA7000血凝仪试剂仓试剂放置时间对血凝四项检测结果的影响

本研究旨在探讨Sysmex CA7000血凝仪试剂仓开封试剂放置不同时间对血浆凝血酶原时间（PT）、纤维蛋白原（FIB）、活化部分凝血活酶时间（APTT）及凝血酶时间（TT）检测结果的影响。采用凝固法采集21例患者凝血标本,使用Sysmex CA7000型全自动血凝分析仪及其原装配套试剂及放置一定时间的试剂和新鲜试剂测定每1例标本的PT、APTT、TT及Fib。各试剂放置时间分别为Thromborel S（TS）：12、24、36、48、60、72 h;Dade Actin（DA）：24、48、72、96、120 h;Test Thrombin（TT）：2、4、6、8、10、12 h;Thrombin Reagent（TR）：4、8、12、16、20、24 h;Owren＇s Veronal Buffer（OVB）：1、2、3、4、5、6 h。以试剂仓放置一定时间为各试验组,以其新鲜试剂（0 h,即时配置）为对照组,计算各试验组PT、APTT、TT及Fib检测值与其对照组间统计学差异,分析放置时间对检测结果的影响。结果表明,当TS、DA、TT、TR及OVB在试剂仓分别放置≥48、96、10、16、4 h时,其PT、APTT、TT及Fib结果与新鲜试剂差异均有统计学意义（P〈0.01）;当放置时间分别达到48-72、96-120、10-12、16-24及4-6 h时,其检测结果与0h间差异百分比分别在-2.6%-10.8%、-3.44%-4.8%、-3.9%-5.52%、-10.8%-3.3%及-17.2%-0.5%之内,其中TS对PT及OVB对Fib影响较为明显;随着试剂放置时间的延长,PT、APTT、TT均呈现延长趋势,而Fib呈现下降趋势。结论：常规检测血凝四项时,需及时更换试剂仓试剂,以保证标本检测结果的准确性。

Influence of Reagent Storage in Sysmex CA7000 for Different Time on 4 Test Results of the Plasma Coagulation

The purpose of this study was to investigate the influence of blood coagulation reagents stored for different time on test results of the specimens prothrombin time（PT）,activated partial thromboplastin time（APTT）,thrombin time（TT）and fibrinogen（Fib）.A total of 21 patient plasma specimens was taken and measured for homeostasis by Sysmex CA7000 automated blood coagulation analyzer and supporting reagent.The PTAPTTTT and Fib of specimens were measured with the reagents stored in Sysmex CA7000 for different time.The differences of PTAPTTTT and Fib were analyzed between values measured of the reagents stored for 0 hour and different time（ TS1236486072 hDA24487296120 hTT2,1012 hTR4, 121620hOVB1 h）, respectively.The results showed that when coagulation reagent TS were stored for more than 48 h,DA 96 hTT 10 hTR 16 h and OVB4 hthe values of PTAPTTTT and Fib of samples were statistically different from the values measured with fresh coagulation reagent（ P 〈0.01）, respectively.Compared 0 h with TS stored for 48- 72 DA 96- 120 TT 10- 12TR16- 24 and OVB 4- 6 hthe percentage difference of PTAPTTTT and Fib is in- 2.6% - 10.8%- 3.44% -4.8%3.9% - 5.52%10.8% - 3.3% and- 17.2% - 0.5%the PT and Fib changes were more significant.Accordinglythe result of PTAPTT and TT had a uptrend as the reagent stored in Sysmex CA7000 analyzer for a long timewhile Fib downtrend.It is concluded that the reagents showed be timely replaced when the plasma coagulation test is performed so as to obtain accurate results of examination.