康复训练对脑缺血损伤大鼠血管生成素的影响

目的：研究运动训练能否促进大鼠局部脑缺血再灌注后的功能恢复并从血管生成素及其受体的角度探讨其机制。方法：成年雄性SD大鼠18只，随机分成运动组、静止组、假手术组。每组6只。大脑中动脉闭塞（MCAO）法造模24h后，运动组给予2周的电动跑台训练，每天30min。采用神经行为学评分、脑梗死体积评价神经功能恢复情况；免疫组化法从蛋白水平观察Ang-1、2，Tie-2的表达情况。结果：2周后，运动组的神经行为学评分高于静止组，脑梗死体积减小具有显著性意义，Ang-1、Tie-2的蛋白表达显著高于静止组与假手术组。结论：运动训练能够促进脑缺血大鼠神经功能恢复．这种改变可能与Antg／Tie-2通路的上调有关。

The effect of rehabilitation training on angiopoietin in ischemia brain injury rats

Objective:To determine if exercises could induce expression of angiopoietin/Tie pathway , in association with angiogenesis; and if angiogenic changes correlated with reduced brain injury in stroke. Method:Adult male Sprague Dawley rats (2.5 month old, n=18) exercised on a treadmill 30min each day for 2 weeks, or housed as non-exercised controls for 2 weeks. Result: Exercises increased protein (determined by immunohistochemistry ) expression of angiopoietin-1 and Tie-2 significantly at the end of 2 weeks(P<0.05). In two-week-exercising rats subjected to 1-h MCA occlusion, neurological deficits and infarct volume reduced significantly. Conclusion:Exercises can reduce brain injury in stroke. The reduced damage is associated with angiogenesis, possibly induced by angiogenic factors following exercises. Physical exercises up-regulates protein levels of the angiopoietin/Tie-2 pathway.