黄芪血清药理对HDF细胞p16mRNA表达及端粒长度影响的实验研究

目的：观察黄芪血清对人胚肺二倍成纤维（HDF）细胞超氧化物歧化酶（SOD）活性、p16mRNA表达及端粒长度影响。方法：以黄芪水煎剂灌胃家兔获得含药血清，以含黄芪血清的DMEM培养基培养HDF细胞，同时以不含黄芪的家兔血清作空白对照，比色法检测HDF细胞的SOD活性、RT-PCR检测P16mRNA表达及TRF-Southem blot法检测HDF细胞端粒长度变化。结果：含黄芪血清培养基培养的HDF细胞与空白血清培养的HDF细胞比较SOD活性增加，P16mRNA表达减少，端粒酶长度增加。结论：含黄芪血清的培养基通过增加HDF细胞SOD活性．减少P16mRNA表达，抑制端粒缩短而起到抗衰老作用。

Effects of astragalus root on the expression of P16 mRNA and telomere of human dermal fibroblasts by serum pharmacology

Objective: To observe effects of astragalus root on the changes of the activity of SOD, the expression of P16 and the length of telomere. Method: The serum of rabbits which were filled with water decoction of astragalus root and then cultured human dermal fibroblasts in DMEM contained with astragalus root serum was collected. The activity of SOD was determined by colorimetric method,the expression of P16 by RT-PCR and the length of telomere by TRP-Southern blot. Result: Compared with those in the control group, the activity of SOD increased, the expression of P16 depressed,the length of telomere lengthened. Conclusion: Astragalus root serum can postpone senescence by increasing the activity of SOD,depressing the expression of P16 and inhibiting the shortening of telomere.