雷公藤多甙对慢性脑低灌注大鼠学习记忆能力的影响

目的：观察雷公藤多甙对慢性脑低灌注大鼠学习记忆能力的影响。方法：SD大鼠随机分为正常组、假手术组、药物对照组、模型组、生理盐水组、雷公藤多甙低、高剂量组。采用永久性结扎双侧颈总动脉制成慢性脑低灌注模型，在模型组的基础上，雷公藤多甙低、高剂量组和生理盐水组分别予以雷公藤多甙10mg·kg-1·d-1、30mg·kg-1·d-1和生理盐水连续灌胃。术后1个月、2个月、3个月时采用Morris水迷宫检测各组大鼠的定位航行潜伏期和空间探索次数。结果：定位航行潜伏期和空间探索次数在术后1个月时各组间差异无显著性意义（P>0.05）；在术后2个月和3个月时模型组和生理盐水组呈进行延长和减少，与假手术组比较差异有显著性意义（P<0.05，P<0.01）。雷公藤多甙低、高剂量组定位航行潜伏期和空间探索次数较模型组和生理盐水组明显缩短和增多（P<0.05，P<0.01），与假手术组比较差异无显著性意义(P>0.05)，雷公藤多甙高剂量组和低剂量组比较差异无显著性意义(P>0.05)。结论：慢性脑低灌注大鼠空间学习记忆能力随观察时间的延长而明显降低，雷公藤多甙能改善慢性脑低灌注大鼠学习记忆能力，这种作用在高剂量和低剂量间差异无显著性意义。

Effect of tripterygium wilfordii polyglycoside on spatial learning and memory ability in a rat model of chronic cerebral hypoperfusion

Objective: To observe the effects of tripterygium wilfordii polyglycoside(TWP) on spatial learning and memory ability of chronic cerebral hypoperfusion in rats. Method: SD rats were randomly divided into normal group, sham operation group, TWP control group, model group, saline group, TWP low dose group and TWP high dose group.The model of chronic cerebral hypoperfusion was established by permanent occlusion of bilateral common carotid arteries(2VO).On the base of the model group, TWP low dose group,TWP high dose group and saline group were treated by gastrointestinal perfusion with TWP at a dose of 10mg/kg ,30mg/kg and saline respectively for one month and two months after 2VO were surgery completed.The escape latencies of oriented sail and the times of spatial explore were examined with Morris water maze. Result: The escape latencies of oriented sail and the times of spatial explore had no difference in all groups at one month after 2VO(P>0.05).But at two and 3 months after 2VO, the escape latencies of oriented sail and the times of spatial explore in TWP low dose group and high dose group were significantly shorter than model group (P<0.05). There was no difference with sham operation group(P>0.05).There was not difference between TWP low dose group and TWP high dose group (P>0.05). Conclusion: Compared with normal rats, spatial learning and memory ability of rats with chronic cerebral hypoperfusion were obviously decreased. TWP can improve the spatial learning and memory ability in a rat model of chronic cerebral hypoperfusion. There was no difference between TWP low dose group and TWP high dose group.