超早期高压氧应用对脊髓完全横断损伤大鼠后肢骨密度的影响

摘要 目的：探讨超早期高压氧应用对脊髓完全横断损伤大鼠后肢骨密度（BMD）的影响。 方法：①55只3月龄健康雌性SD大鼠随机分为假手术组（Sham组，15只）、模型组（CSCT组，20只）及高压氧组（HBO组，20只）。Sham组仅行T10椎板切除术，其余两组均行T10椎板水平脊髓完全横向切断术。②HBO组于术后第3小时置于动物舱内开始高压氧处理，10d为1疗程，共3疗程，其中第一疗程每日2次，第二、三疗程均每日1次，每疗程间休息6d。③术后6周末取材，采用Delphi A型扇形束DEXA骨密度仪检测各组离体股骨、胫骨整体及其不同区域BMD。 结果：CSCT组与Sham组比较，前者股骨整体、股骨远端(FR1，FR2)、近端(FR6，FR7)及胫骨近端(TR1，TR2)BMD均明显下降，差异有显著性意义（P＜0.05—0.01)；HBO组与CSCT组比较，前者股骨整体BMD明显增加（P＜0.05)，而胫骨整体、股骨及胫骨其他各区域BMD均略呈上升趋势，但差异无显著性意义。 结论：超早期高压氧应用可减缓脊髓完全横断损伤大鼠后肢骨丢失。

Effects of ultra-early stage hyperbaric oxygenation on the hindlimb bone mineral density in rats after complete spinal cord transection

Objective:To study the effects of ultra-early stage hyperbaric oxygenation(HBO) on the hindlimb bone mineraldensity(BMD)in rats after complete spinal cord transection(CSCT).Method:Fifty-five SD rats were randomly assigned into three groups:sham-operated(Sham) group, CSCT group,and HBO group. All rats underwent laminectomy at T10, while rats in CSCT and HBO groups also underwent CSCT at the same level. Three hours post operation, HBO group started receiving HBO therapy comprising threecourses of 10-day treatment. BMD of total femur, total tibia and their subregions in vitro was measured with du-alenergy X-ray absorptiometry (DEXA).Result:After 6-week treatment, BMD of total femur, BMD in regions1—2 at the distal femur(FR1,FRZ2), BMDin regions 6—7 at the proximal femur(FR6,FR7) and BMD in regions 1—2 at the proximal tibia(TR1,TR2) invitro decreased markedly in CSCT group at 6 weeks post-operation compared with that in Sham group (P<0.05—0.01). In HBO group, BMD of total femur in vitro was significantly higher than that in CSCT group (P<0.05),whereas BMD of the femur subregions, total tibia, and tibia subregions in vitro tended to be higher than thosein CSCT group, but with out statistical significance.Conclusion:Ultraearly stage HBO could alleviate the hindlimb bone loss in femur bone in rats following complete spinal cord transection.