| 原因不明复发性流产病理妊娠患者外周血CD4+CD25+Treg、Foxp3mRNA的表达 |
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| 引用本文： | 章卫国,张蔚卿,陈葆国,朱敏.原因不明复发性流产病理妊娠患者外周血CD4+CD25+Treg、Foxp3mRNA的表达[J].全科医学临床与教育,2011,9(4):368-371. |
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| 作者姓名： | 章卫国 张蔚卿 陈葆国 朱敏 |
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| 作者单位： | 1. 台州医院中心实验室,浙江临海,317000
2. 台州医院妇科 |
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| 摘 要： | 目的探讨原因不明复发性流产(URSA)病理妊娠患者外周血CD4+CD25+调节性T细胞(Treg)及叉状头/翅膀状螺旋转录因子(Foxp3)mRNA的表达。方法采用流式细胞术测外周血CD4+CD25+Treg,半定量、实时荧光定量PCR分析外周血单个核细胞Foxp3转录水平;检测70例原因不明复发性流产史妇女(URSA未孕组)、20例病理妊娠妇女(URSA病理妊娠组)、22例正常妊娠妇女组(正常妊娠组)、20例正常非孕妇女组(对照组)外周血中CD4+CD25+Treg、Foxp3 mRNA的表达情况。采用酶联免疫吸附法检测血清白细胞介素-10(IL-10)水平。结果 URSA未孕组外周血CD4+CD25+Treg、Foxp3 mRNA、IL-10比例明显低于正常非孕妇女组,两组比较,差异均有统计学意义(t分别=3.61、4.18、2.71,P均<0.05);URSA病理妊娠组外周血中CD4+CD25+Treg、Foxp3 mRNA、IL-10低于正常妊娠妇女组,两组比较,差异均有统计学意义(t分别=3.79、12.56、4.42,P均<0.05)。结论外周血CD4+CD25+Treg、Foxp3 mRNA和IL-10表达水平降低可能与URSA病理妊娠患者的发病有关。
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| 关 键 词： | URSA 病理妊娠 调节性T细胞 Foxp3mRNA IL-10 |
Expression of Foxp3 mRNA in peripheral regulatory T cells of pathological pregnancy patients with unexplained recurrent spontaneous abortion |
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| ZHANG Weiguo,ZHANG Weiqing,CHEN Baoguo,et al..Expression of Foxp3 mRNA in peripheral regulatory T cells of pathological pregnancy patients with unexplained recurrent spontaneous abortion[J].clinical education of general practice,2011,9(4):368-371. |
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| Authors: | ZHANG Weiguo ZHANG Weiqing CHEN Baoguo |
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| Institution: | ZHANG Weiguo,ZHANG Weiqing,CHEN Baoguo,et al.Medical Research Center,Taizhou Hospital of Zhejiang Province,Linhai 317000,China |
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| Abstract: | Objective To observe the frequency of CD4+CD25+ regulatory T cells and expression of Foxp3 mRNA in peripheral of patients with unexplained recurrent spontaneous abortion(URSA) and pathological pregnancy.Methods Seventy URSA patients(URSA group),22 pathological pregnancy patients(pathological pregnancy group),20 healthy pregnant women(normal pregnancy group) and 20 healthy non pregnant women with at least one normal birth(control group) were involved and the frequency of peripheral CD4+CD25+Treg was detected... |
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| Keywords: | unexplained recurrent spontaneous abortion pathological pregnancy regulatory T cells Foxp3 mRNA IL-10 |
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