克骨汤对兔激素性股骨头坏死血管内皮生长因子表达影响的实验研究

目的观察克骨汤对兔激素性股骨头坏死血管内皮生长因子(VEGF)表达的影响,探讨其作用机制。方法 36只新西兰白兔随机分为三组:模型+克骨汤组(简称克骨汤组)12只、模型组12只、对照组12只。在第4、8、12周分批处死实验动物,获取支配股骨头血运的主要血管(旋股内侧动脉、股静脉)及股骨头。将其制成标本,采用原位杂交法测定VEGF含量,并行常规病理组织学检查。结果第4周时,各组动脉内膜层、静脉内膜层中都有VEGF mRNA阳性染色,但各组间的差异无统计学意义(F分别=1.36、2.46,P均>0.05);第8周和第12周时,各组VEGF mRNA阳性染色的差异有统计学意义(F分别=14.58、11.35,P均<0.05);第8周时,与克骨汤组、对照组比较,模型组动脉内膜层、静脉内膜层的VEGF mRNA阳性染色减弱,差异均有统计学意义(t分别=12.13、15.23;19.36、18.25,P均<0.05);第12周时,克骨汤组与模型组、模型组与对照组比较,动脉内膜层、静脉内膜层VEGF mRNA阳性染色差异均有统计学意义(t分别=12.09、19.35;20.37、16.74,P均<0.05),但克骨汤组与对照组比较,静脉内膜层VEGF mRNA阳性染色差异无统计学意义(t=2.59,P>0.05)。结论克骨汤可上调VEGF mRNA在血管内膜中的表达,使兔激素性股骨头坏死血管壁中主要促血管生长因子生理活性增强。

Effect of Kegu recipe on angiogenesis stimulating factors in the steroid-induced avascular necrosis of the rabbit femoral head

Objective To investigate the effect of Kegu recipe on angiogenesis stimulating factors （VEGF mRNA）in the steroid-induced avascular ecrosis of the rabbit femoral head. Methods A total of 36 adult New Zealand rabbits were randomly divided into three groups equally： the control group, the model group, and the Kegu recipe group. The experimental animals were partial executed at fourth, eighth, twelfth weeks so that to access the main blood vessels of the femoral head including the medial femoral circumfles artery, femoral medial femoral circumflex artery and femoral vein. The specimen used the in situ hybridization method to measure VEGF levels and underwent routine histologic examination. Results At fourth week, there was no statistical significance between three groups though all groups showed positive staining in VEGF mRNA of endarterium and vein intima（F=1.36,2.46,P〉0.05）. At eighth and twelfth week, the differences of VEGF mRNA positive staining in endarterium and vein intima were statistical significance （F=14.58,11.35,P〈0.05）. At eighth week, the VEGF mRNA positive staining in endarterium and vein intima of model group was significantly lower than Kegu recipe group and control group（t=12.13,15.23,19.36,18.25,P〈0.05）. At twelfth week, the differences of the VEGF mRNA positive staining in endarterium and vein intima were statistical significance between model group and control group as well as between Kegu recipe group and model group（t=12.09,19.35,20.37,16.74,P〈0.05）. The differences of the VEGF mRNA positive staining in vein intima between Kegu recipe group and control group had no statistical significances （t=2.59,P〉0.05）. Conclusions Kegu recipe could improve the presence of VEGF mRNA, which may boost the biological activity of the major factors for angiogenesis in the vascular wall of the steroid-induced necrosis of the femoral head.